LOCUS       sequence-129053-         850 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_662_S2-1-Tor_dCP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    234..258
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(755..779)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(825..844)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccgttt ggtatgcgtc ttgtgattca aagttggctt attcaaagga tattaacgaa
      121 ggcagcggca cgtctgctcg gctcgaacat tcattcacaa ctgatgctct cagccacccc
      181 gcgccctttt ataccgctgc gctcgatccc cgggcgagct cgatcgcaga tctaccactt
      241 tgtacaagaa agctgggtcg aattcgccct tgcccgggtt ataaaataca aaattcgaaa
      301 atagcacaac gcttggcttt cgtagctaaa catcgaaaac gaaatccaaa aacaaagact
      361 caaagcaccc tgtagcccgg caattgtgcg cagttacccc aacaactaca cacatttttc
      421 aaacgacgaa caaaagataa tgtcgacaac taaaccgcag atatgcatat gtttatgtac
      481 tccgtacaag tcgctgtact acgcacatac acacatagta tatttaacaa aacggagcca
      541 agtcgcggaa gtaattctcc gcatcttatc gcacttagag attccgcccc acaatccaaa
      601 tccatcccgc cggagtactg tcctccgagt gcagactgcg tgccagatct ggccgtatgt
      661 atatcgtcta aattgatatt agcggacgag agctacttga cactatttct cagtggctgt
      721 cacttcacag aaattgtgca aagggcgaat tcggagcctg cttttttgta caaacttgtg
      781 gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca gcctattttg
      841 ctagtttgtt
//
