LOCUS       sequence-129057-         876 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  The heterotrimeric G-protein subunit, G-gamma-2, tagged with 
            circular permutated Venus (cp173V)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: cpVenus-G-gamma-2
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..780
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     183..204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(307..328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(568..587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgga cggcggcgtg
       61 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      121 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      241 tacaagggtg gcagcggtgg catggtgagc aagggcgagg agctgttcac cggggtggtg
      301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      361 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
      421 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
      481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      541 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      601 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      661 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      721 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      781 tccggactca gatctatggc cagcaacaac accgccagca tagcacaagc caggaagctg
      841 gtagagcagc ttaagatgga agccaatatc gacagg
//
