LOCUS       sequence-129126-         931 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, G 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: dTomato_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(159..872,873..875,876..878))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     812..833
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggaaccttta atagaaattg gacagcaaga aagcgagctt agtgatactt gtgggccagg
       61 gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattgc
      121 tcgaggttta aacagctcca ccgcggtggc ggccgctatt acttgtacag ctcgtccatg
      181 ccgagagtga tcccggcggc ggtgcggaac tccagcagga ccatgtgatc gcgcttctcg
      241 ttggggtctt tgctcagcac ggactgggtg ctcaggtagt ggctgtcggg cagcagcacg
      301 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
      361 tccacgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gacggccatg
      421 atatagatgt tgtggctgtt gaagttgtac tccagcttgt gccccaggat gttgccgtcc
      481 tccttgaagt cgacgccctt cagctcgatg cggttcacta gggtgtcgcc ctcgaacttc
      541 acctcggcgc gggtcttgta ggtgccgtcg tccttgaaga agatggtgcg ctcctggacg
      601 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      661 gcgaagcact gcacgccgtg gctcagggtg gtcacgaggg tgggccaggg cacgggcagc
      721 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt tggtggcatc gccctcgccc
      781 tcgcccctca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      841 accaccccgg tgaacagctc ctcgcccttg ctcaccatag agccaccacc ggagcctccg
      901 ccggtacctc caccgtactc gtcaattcca a
//
