LOCUS       sequence-12919-e       12257 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR, 
            Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L, 
            Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E, 
            Dallapiccola B, Gleeson JG
  TITLE     Mutations in CEP290, which encodes a centrosomal protein, cause 
            pleiotropic forms of Joubert syndrome.
  JOURNAL   Nat Genet. 2006 Jun . 38(6):623-5.
  PUBMED    16682970
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2006 Jun . 38(6):623-5."
COMMENT     Sequence Label: FLAG-mCep290
FEATURES             Location/Qualifiers
     source          1..12257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             932..976
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             977..1000
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2789..2800
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8587..9209
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8786..8805)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(9233..9253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9238..9567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9418..9553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9605..9624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9606..9624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9638..9654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9638..9654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9651..9673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9662..9678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9686..9692,9693..9710,9711..9716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9731..9752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9869..9886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10040..10628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10120..10139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10799..11590,11591..11659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11422..11441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11660..11764)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11791..12246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11878..11897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12088..12109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctgact acaaagacga tgacgacaag ctgcggccgc cagtgggatg
     1021 gatatctgca gaattcgccc ttatgccacc taatataaag tggaaagaat taatcaaagt
     1081 tgatccagat gacctgccac ggcaagaaga gttagcagat aaattattga tatctttatc
     1141 caaggtggaa gtaaatgaac taaaaaatga agaccaagaa aacatgatac atctattcag
     1201 aattacccag tctctaatga agatgaaagc ccaggaagta gagctcgctt tggaagaagt
     1261 tgaaaaggct ggagaagaac aagcaaaatt tgaaaatcaa ttaaagacaa aagtaatgaa
     1321 actggaaaat gaactggaga tggctcagca gtctgcaggg ggacgtgaca ctcggttttt
     1381 acgtgacgaa attcgccaac ttgagaagca gctggaacaa aaagatagag aattagagga
     1441 tatggaaaaa gaattggata aagaaaagaa ggttaatgaa caattggctc ttcgaaatga
     1501 ggaggcagaa aatgaaaaca gcaaattaag aagagagaat gagcagcttc ggcaggacat
     1561 tattgactac cagaaacaaa tagattcaca gaaagaatca cttctgtcaa ggagaggaga
     1621 agacagtgac taccgatcac agttgtctaa aaagaactat gaacctgttc aatatctgga
     1681 cgaaatacag accttaacag aagctaatga gaagattgaa gttcagaacc aagaaatgag
     1741 gaaaaacctg gaagagtctg tgcaggagat ggagaagatg actgatgagt acaacaggat
     1801 gaaagcgctt gtgcatcagt cggatgctgc catggaccag atcaagaagg agaatgagca
     1861 ctatcgcctg caagttcgag agctcacgga tcttctgaag gcgaaggatg aagaggacga
     1921 tccagtcatg atggctgtta acgcaaaagt ggaagagtgg aagttaattt tgtcttctaa
     1981 agatgatgaa atcattgaat atcagcaaat gctacagagt ctgagaggga aacttaaaaa
     2041 tgcccaactt gatgcagaca aaagtaacat catggctctg aaacagggta tccaggagcg
     2101 agacagtcaa attaagatgc ttactgagca agtagaacag tatacgaaag aaatggaaaa
     2161 aaatacgttt attattgaag atttgaaaaa tgaactccaa aaagacaaag gtacttcaaa
     2221 cttttatcag cagactcatt atatgaaaat tcactcaaaa gtacaaattt tagaagagaa
     2281 aacaaaagag gccgagagaa tagctgagct ggctgaggct gatgccaggg agaaggacaa
     2341 agaattggtt gaggctctga agagattaaa agattatgaa tctggagtat atggcttaga
     2401 agatgctgtt atcgaaataa agaattgtaa agcccaaatt aaaataagag atggagagat
     2461 ggaagtgttg accaaggaga tcaataagct tgagatgaag atcaatgaca tccttgatga
     2521 aaacgaagcc cttagagagc gggctggcct tgaacccaag acaatgattg atttgactga
     2581 atttagaaac agcaaacggt taaagcagca acagtacaga gctgagaacc aggttctttt
     2641 gaaagagatc gaaagtctag aggaggagcg tcttgacttg aaaagaaaga ttcgtcaaat
     2701 ggctcaagaa agaggcaaaa ggaacgcagc ctcaggatta accattgacg acttgaactt
     2761 atctgaaacc ttttctcatg aaaataaaat agaaggaaga aaactaaatt ttatgagcct
     2821 caataatatg aatgaaacac aatcaaagaa tgagtttctt tcaagagaat tggctgaaaa
     2881 ggaaaaagat ttagaaagaa gtaggacagt aattgctaaa ttccagagta aactaaaaga
     2941 attagtcgaa gaaaataagc aacttgaaga aggtatgaaa gaaattttgc aagctattaa
     3001 ggacatgccg aaggattctg atgtgaaagg aggtgaaaca tctttaatca tcccgagtct
     3061 tgaaagactg gttaacgcta tggaatcaaa gaatgcagaa ggaatcttcg atgccagctt
     3121 gcatctaaaa gcccaagttg accaacttac aggaaggaat gaagaattaa ggcaagaact
     3181 caggcaatct cggaaagagg ctgtaaatta ttcacagcag ttggtaaaag caaacttaaa
     3241 gattgaccac cttgagaagg aaactgacct tctacgtcag tctgcaggct ccaatgtagt
     3301 atacaaaggc atagacttac ccgatgggat agcaccatcc agtgcctata tcatcaattc
     3361 tcagaatgaa tacttaatac atcttttgca ggaactagac aataaagaaa agaaattaaa
     3421 acatttagaa gattcccttg aagattataa cagaaagttt gcagtgattc gtcatcaaca
     3481 aagtttatta tataaagaat acctaagtga aaaggatatt tggaaaacag actctgagat
     3541 gataagagag gagaagagaa aactggaaga tcaagctgag caggatgctg tgaaagtaaa
     3601 agagtacaac aatttgctca gtgctcttca gatggattcg aacgagatga agaaaatgct
     3661 ctcagaaaac agtaggaaaa tcaccgttct gcaagtgaac gagaagtccc tcatccggca
     3721 gtacaccacc ttagtggaga tggagcggca cctgagaaag gaaaacggga aacacaggaa
     3781 tgacgtcata gccatggaag ccgaagtcac tgaaaaactt ggaagtttgc aaagattcaa
     3841 ggaaatggcc atcttcaaga ttgcagctct tcagaaggtt atagataata gtgtttcttt
     3901 gtctgaatta gaactagcca ataaacagta caatgagctg actactaagt acagggacat
     3961 cttgcagaaa gataatatgc ttgttcaaag aacaagtaac ttagaacacc tggagtgtga
     4021 gaatgcatcg ctaaaagaac agatggaggc tatcagtaaa gagctggaga ttacaaagga
     4081 aaaactccat accattgaac aggcctggga acaagaaacg aagttaggaa atgactcaaa
     4141 catggataag gctaagaaat caatgacaaa cagtgacatt gtttctattt caaagaaaat
     4201 cactgtgttg gagatgaagg aattaaatga aaggcagagg gctgaacact gtcagaaaat
     4261 gtatgagcac ttaaggactt cattaaagca aatggaagaa cgtaattttg aattggaaac
     4321 caagttcact gagcttacta aaatcaacct ggatgcacaa aaggtggagc agatgttgag
     4381 agacgaatta gctgatagtg tgaccaaggc agtaagtgat gctgaccgac agcggattct
     4441 agaactagag aagagtgaag tggagctcaa agttgaagtg tccaagctga gagagatttc
     4501 agatattgcc aaaagacaag ttgatttttt gaattcgcaa caacagtcca gggaaaagga
     4561 agtggaatcc ctcagaacgc agctgctgga cttccaggca caatctgacg aaaaggctct
     4621 aattgccaaa ttgcaccaac atgttgtctc tcttcaaatt agtgaggcca ctgccctcgg
     4681 taagttagag tcagttacgt ccaaactcca gaagatggaa gcctacaatt tgcgcttaga
     4741 acagaaactg gatgaaaaag agcaggcgct ctactatgct cgtttggaag gtagaaacag
     4801 agcaaaacac ctgcgccaaa ccattcagtc gcttcgaaga cagttcagtg gagctctacc
     4861 cttagcacag caggaaaagt tctccaaaac gatgattcag ttgcaaaatg acaaacttaa
     4921 gataatgcaa gaaatgaaga attcgcaaca ggaacacaga aatatggaaa acaaaacact
     4981 ggagttggaa ttaaaattaa aaggcttaga agaattgatc agtactttaa aggatgccag
     5041 gggagcccag aaggtaatca attggcatgt gaaaatagaa gaacttcgcc tccaagaact
     5101 taagctaaat agagaactag tcaagggtaa agaagaaatc aaatatttga ataatatcat
     5161 ctctgaatat gagcatacaa tcaacagtct agaggaagaa attgttcagc aaagcaagtt
     5221 ccatgaagaa agacagatgg cttgggatca aagagaagtt gagctggaac gccagttaga
     5281 catttttgat catcagcaaa atgaaatact cagtgcagca caaaagtttg aagactctac
     5341 aggatcaatg ccagacccca gcttgcctct tccaaaccaa cttgaaattg ctctaagaaa
     5401 aattaaggag aatattcaag taattcttaa aacacaagca acttgcaagt cactagaaga
     5461 gaaactaaaa gaaaaagaat ctgctttacg gttggcagag caaaatattc tgtcaagaga
     5521 caaagtaatc aatgaactga ggcttcgatt gcctgccacg gctgatcgag aaaaacttat
     5581 agctgagcta gaaagaaaag agctggagcc gaaatctcat cacacaatga aaattgccca
     5641 ccaaactatt gccaacatgc aggcaaggtt aaatcacaag gaagaagtat tgaagaaata
     5701 ccagcacctt ctggagaagg ccagagagga gcaaagagaa attgttaaga agcatgagga
     5761 agaccttcat gttcttcatc acaaattaga acaacaggcc gataattcac tcaataaatt
     5821 cagacagaca gctcaggatt tacttaagca gtctcctgct ccagttccca ccaacaaaca
     5881 tttcattcgt ctggccgaga tggagcagac agtagcagaa caagatgact ctctgtcctc
     5941 acttttgacc aaactaaaga aagtatcaaa agatttggaa aaacaaaaag aaatcactga
     6001 gttaaaagtc agagagtttg aaaataccaa actacggctc caagaaactc atgccagtga
     6061 ggtaaagaaa gtgaaagcag aggtagagga cttaaggcat gctctagccc aagcacacaa
     6121 ggactcccag agtttaaagt ctgaactcca ggctcagaaa gaagcaaact ccagagctcc
     6181 aacaaccaca atgaggaatc ttgtagacag gctaaagagc caactagcct tgaaagagaa
     6241 gcaacaaaag gcacttagtc gagccctgtt ggaacttcgg tcggaaatga cagcagcagc
     6301 tgaggaacgt ataatcgctg taacttctca aaaagaggca aatctcaatg ttcaacaagt
     6361 tgttgagcgc catactagag agctaaagtc acaaattgaa gatttaaatg aaaatctttt
     6421 aaaattgaaa gaagctctta aaacaagtaa gaacaaagaa aattcactag ctgatgattt
     6481 aaatgaatta aataatgaac tgcaaaaaaa gcaaaaagct tataataaaa tccttagaga
     6541 gaaagatgga attgatcaag aaaatgatga actgagaaga cagattaaaa gactgtccag
     6601 tggactgcag agcaaaactt tgatagataa caagcaaagt ttaatcgatg aacttcaaaa
     6661 gaaagttaaa aaacttgaaa gccaactgga aagaaaggtg gatgacgtag acataaagcc
     6721 ggtgaaggaa aagagtagta aagaagaatt aattaggtgg gaagaaggta agaaatggca
     6781 aaccaaagta gagggactac gaaacagact aaaggagaag gaaggagaag cccacggcct
     6841 ggcaaagcag ctgaatacct taaaggaact ttttgccaaa gctgataaag agaaacttac
     6901 tttgcagaag aaactgaaaa caacaggaat gactgttgac caggttttag gagtgcgagc
     6961 tttggaatct gaaaaagagt tggaagagct aaaaaagaaa aatctggacc tagaaaatga
     7021 catattatac atgaggaccc agcaggctct tccacgagat tctgttgtgg aagacttaca
     7081 tttacaaaat aaataccttc aagaaaaact tcatacttta gaaaaaaaac tttcaaagga
     7141 gaaatattct cagtctttga cttcagaaat agagtcagat gatcactgtc aaaaagaaca
     7201 agaacttcag aaggaaaatt tgaagttgtc atctgaaaac atcgagctga aatttcaact
     7261 tgaacaagca aataaagatt tgccaagact aaagaatcaa gtgaaagatt tgaaggaaat
     7321 gtgtgaattt cttaagaaag gaaaactgga acttgagcgg aagcttggtc aggtcagagg
     7381 ggctggtaga agtgggaaga caatcccaga actagaaaaa accattgggt taatgaagaa
     7441 agtagttgaa aaagtccaaa gagaaaatga acaattgaaa aaggcatcag gaatactgac
     7501 tagtgaaaaa atggctacta ttgaggaaga aaatagaaac ttaaaggctg aactagaaaa
     7561 gcttaaagct cactttggac gtcagttgag tatgcagttt gaatctaaga acaaaggtac
     7621 tgagaaaatt gttgccgaaa atgaacggct tcggaaagaa cttaagaaag aaatagaagc
     7681 ctctgagaaa ctgcggatag ctaagaacaa cttagagctg gtgaacgaca agatggcagc
     7741 tcaactcgaa gaaactggga agagactaca gtttgcagaa agtagagccc cacagctgga
     7801 aggtgctgac agcaagagct ggaagtcaat tgtggtctca agagtgtatg agaccaagat
     7861 gaaagagctt gaaagtgaca ttgccaaaaa gaatcaaagt atcactgacc ttaaacagct
     7921 tgtaagagaa gcaacagaga gagaacagaa agctaagaaa tacactgaag accttgaaca
     7981 acagattgag atcctcaaaa atgttcctga aggtgccgag acagagcaag agcttatacg
     8041 ggaactccag cttcttagat tagccaataa tcagatggat aaagaaaggg cagaattaat
     8101 ccatcagata gaaattaaca aggaccaaac cagagctgac agtagcatac ctgattctga
     8161 tcaactaagg gaaaagataa atgacctgga gacacaactc agaaagttgg agctagaaaa
     8221 gcaacattcg aaggaggaag ttaaaaagct gaaaaaagaa ctggaaaatt ttgatccttc
     8281 attttttgaa gaaattgaag acctgaagta taattataag gaagaagtga aaaagaatat
     8341 cctattagaa gagaagctaa aaaaactgtc ggaacagttt ggatttgaac tgcctagtcc
     8401 tcttgctgct tctgaacact cggaagatgg agaaagtcct catagtttcc ctatttatta
     8461 gctccctcgt gtaactgcag gtttaaaact attttatgag tgaaaaaagt ttcagctgga
     8521 aatcaagcag tctctgtgtt ataagggcga attccagcac actggcggcc gttactagtg
     8581 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     8641 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     8701 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     8761 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     8821 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     8881 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     8941 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     9001 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     9061 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     9121 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     9181 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg
     9241 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9301 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9361 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9541 ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt
     9601 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
     9661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11761 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
    11821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    11881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    12181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    12241 caatttccat acaattt
//
