LOCUS       sequence-129326-         338 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-FLAGHISNDHFR
FEATURES             Location/Qualifiers
     source          1..338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..29
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agggatctag ccatcatcat catcatcaca gcagcggatc tggtggtggt ggtggtgaaa
       61 acctgtactt ccagggatcc atcagtctga ttgcggcgct agcggtagat cgcgttatcg
      121 gcatggaaaa cgccatgcca tggaacctgc ctgccgatct cgcctggttt aaacgcaaca
      181 ccttaaataa acccgtgatt atggggcgcc atacctggga atcaatcggt aggcctttgc
      241 ccggccgcaa aaatattatc ctcagcagtc aacccgggac cgatgatcgg gttacctggg
      301 ttaaatcggt cgacgaagcc atcgcggcgt gtggtgac
//
