LOCUS       sequence-129390-        1174 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_720_S2-1_CGCG-to-ETS21C
FEATURES             Location/Qualifiers
     source          1..1174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(539..563)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      754..763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(826..844)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccagaacatt tctctatcga taggtaccac aagtttgtac aaaaaagcag gctccgaatt
       61 cgccctttgc acaatttctg tgaagtgaca gccactgaga aatagtgtca agtagctctc
      121 gtccgctaat atcaatttag acgatataca tacggccaga tctggcacgc agtctgcatt
      181 cccaatgatg cgggatggat ttggattgtg gggcggaatc tctaagtgcg ataagatgcg
      241 gagaattaac cggaaattgg ctccgttttg ttaaatatac tatgtgtgta tgtgcgtagt
      301 acagcgactt gtacggagta cataaacata tgcatatctg cggtttagtt gtcgacatta
      361 tcttttgttc gtcgtttgaa aaatgtgtgt agttgttggg gtaaccggaa atattgccgg
      421 gctacagggt gctttgagtc tttgtttttg gatttcgttt tcgatgttta gctacgaaag
      481 ccaagcgttg tgctattttc gaattttgta ttttataacc cgggcaaggg cgaattcgac
      541 ccagctttct tgtacaaagt ggtagatctg cgatcgagct cgcccgggga tcgagcgcag
      601 cggtataaaa gggcgcgggg tggctgagag catcagttgt gaatgaatgt tcgagccgag
      661 cagacgtgcc gctgccttcg ttaatatcct ttgaataagc caactttgaa tcacaagacg
      721 cataccaaac ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
      781 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
      841 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      901 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
      961 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     1021 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     1081 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     1141 gtttccaaaa aggggttgca aaaaattttg aacg
//
