LOCUS       sequence-129444-        1036 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_240_pAGW_V5
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..58
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             68..508
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    530..654
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(679..684,685..702,703..709)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(829..848)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggatccagat atcatgggga agccaatccc taatcccctt ctgggactcg actctaccgg
       61 cggctctatg aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa
      121 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
      181 tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga
      241 atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga
      301 catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt
      361 acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat
      421 gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa
      481 caaaggtcaa agacagttga ctgtatcgtc gggcggcggc caccggtata caagtttgta
      541 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      601 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
      661 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      721 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      781 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      841 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      901 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      961 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1021 agtgttcacc cttgtt
//
