LOCUS       sequence-129552-         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expession of Flag-CtlP T847E mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pCW-Flag-CtIP-T847E
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc
       61 ccaaccccgg atccttagtg gtggtggtgg tggtggaccg gttcattact aaaccacttt
      121 gtacaagaaa gctgggtcct atgtcttctg ctccttgcct tttggagaaa atattgcgtt
      181 gtaaggctga cgtctttttg gacgaggaca aggatcaaga tcttccttaa tataacctct
      241 ttccatacaa gtctgagtgg aaggaaaacc aacttcccaa aaattctctg gctcgttggg
      301 tggaatgtag cggaatcggt gtcttgagca ggaagccaat ttcttttctc tttcttctgc
      361 tggcatatct gcataataaa tttcacattc cttacacgtg tgcccaagca gttttcttct
      421 ctcctctttt ttccgaacca cctcaatatg aggaaaattt tgcaagctag tctctctttc
      481 atcaccttta aaatacggct ccacaaacgc tttctgcttg actttgtctt gtttatcacc
      541 atgagtgtgt agtttctttg tggcagtaga caattcctcc tcttcatctg ctgcttggga
      601 gaaactgtct gccaaacagg attcatactc ttcatgtgtt gtccgatcaa acatatcttc
      661 caagctatca ttcatttttc tttcttcatt tgaacttttt tctcccttct gctcttgttt
      721 cttcattttt aagagaacgg tttcactaac caatgtacag tccatgtcta ctgtctctcc
      781 tcctaatttt gactgactgc catcctttgt atctattaca gtaacatcca tt
//
