LOCUS       sequence-129588-        1113 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Protein production from a synthetic gene encoding Treponema 
            denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Tranchimand S, Starks CM, Mathews II, Hockings SC, Kappock TJ
  TITLE     Treponema denticola PurE Is a bacterial AIR carboxylase.
  JOURNAL   Biochemistry. 2011 May 31;50(21):4623-37. doi: 10.1021/bi102033a. 
            Epub 2011 May 6.
  PUBMED    21548610
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi102033a";
            journalName: "Biochemistry"; date: "2011-05-31- 31"; volume: "50"; 
            issue: "21"; pages: "4623-37"
COMMENT     Sequence Label: pJK376
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     679..698
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             729..751
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
       61 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      121 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      181 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      241 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
      301 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
      361 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
      421 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
      481 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
      541 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
      601 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
      661 atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
      721 gaaataattt tgtttaactt taagaaggag atatacatat gcgtccgctg gtgattatcc
      781 ttatgggcag cagttcagat atgggtcacg ccgagaaaat tgcgtctgaa ttgaaaactt
      841 ttggtattga gtatgccatt cgcatcggct ccgcgcataa aacagcggag catgtagtgt
      901 ctatgctgaa agaatatgaa gctttggacc gtcctaaact gtacatcacg atcgctgggc
      961 gtagtaacgc tttatcaggc tttgttgatg gcttcgtcaa aggtgcaacc attgcctgcc
     1021 caccgccaag cgatagcttc gccggcgccg acatttattc ttcgttacgg atgccgagcg
     1081 ggattagtcc ggcactggtt cttgaaccga aaa
//
