LOCUS       sequence-129759-        1048 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses miR-26a-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Chang TC, Yu D, Lee YS, Wentzel EA, Arking DE, West KM, Dang CV, 
            Thomas-Tikhonenko A, Mendell JT
  TITLE     Widespread microRNA repression by Myc contributes to tumorigenesis.
  JOURNAL   Nat Genet. 2008 Jan;40(1):43-50. Epub 2007 Dec 9.
  PUBMED    18066065
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2008-01"; volume: "40(1)"; pages: "43-50. Epub 2007 
            Dec 9"
COMMENT     Sequence Label: pMSCV-PIG-miR-26a-2
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        380..879
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(415..438)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     812..831
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(900..919)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgagcggcag ggtgtctgtc
       61 tagtctatgg tcattgaggg gaaaaagtca cttctccctg gtgcaattca ttacctaatc
      121 atgacctgga cagactgtcc tgtcggagcc aaggacagaa agctcccata gaggctgtgg
      181 ctggattcaa gtaatccagg ataggctgtt tccatctgtg aggcctattc ttgattactt
      241 gtttctggag gcagctgatg gtccgccgcc ggaaacagag atggctcctg ggacatggtg
      301 tgtgcgcttc ttcctgagcc aggttgaggt tgggaccact gatccattgg aagcctgctc
      361 gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
      421 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
      481 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
      541 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
      601 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
      661 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
      721 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
      781 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
      841 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
      901 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
      961 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     1021 gccacatcga gcgggtcacc gagctgca
//
