LOCUS       sequence-130042-        1046 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  luciferase reporter with mouse Id1 promoter fragments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Lewis TC, Prywes R
  TITLE     Serum regulation of Id1 expression by a BMP pathway and BMP 
            responsive element.
  JOURNAL   Biochim Biophys Acta. 2013 Oct;1829(10):1147-59. doi: 
            10.1016/j.bbagrm.2013.08.002. Epub 2013 Aug 13.
  PUBMED    23948603
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagrm.2013.08.002"; journalName: "Biochim Biophys Acta";
            date: "2013-10"; volume: "1829"; issue: "10"; pages: "1147-59"
COMMENT     Sequence Label: p4XBRE-SBE-SV40-GL3
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(75..271)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      89..224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    389..480
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(421..440)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    494..542
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(810..831)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1022..1041
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagctacc
       61 ggaatgccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
      121 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga
      181 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
      241 gggactatgg ttgctgacta attgagatgc agatcgcaga tcttcagacc gttagacgcc
      301 aggccctcag accgttagac gccaggccct cagaccgtta gacgccaggc cctcagaccg
      361 ttagacgcca ggcccggtac ctatcgatag agaaatgttc tggcacctgc acttgcactg
      421 gggacagcct attttgctag tttgttttgt ttcgttttgt tttgatggag agcgtatgtt
      481 agtactatcg attcacacaa aaaaccaaca cacagatgta atgaaaataa agatatttta
      541 ttgcggccgc tccaagtacc tcccgtacct taatattact tacttatcat ggtagcttgg
      601 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      661 atggcgaatg gcaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
      721 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      781 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
      841 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
      901 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
      961 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1021 aagggaagaa agcgaaagga gcgggc
//
