LOCUS       sequence-130050-         735 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  RSF1010-based vector with extensive polylinker, and resistance to Cm
            and Em. Shuttle vector suitable for transfer to, and replication in,
            E. coli and Anabaena (Nostoc) sp. strain PCC 7120..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch
            E
  TITLE     Paired cloning vectors for complementation of mutations in the 
            cyanobacterium Anabaena sp. strain PCC 7120.
  JOURNAL   Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.
  PUBMED    17639350
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2007-12"; volume: "188(6)"; pages: "551-63. Epub
            2007 Jul 17"
COMMENT     Sequence Label: pRL1342
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..157
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(319..338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(320..338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    354..387
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgagcgg ccgcatacga tttaggtgac actataggat ccctgccggc cggtccggcg
      181 cgcccgggct cgagtgcacg tacgacgtct agacctaggc cttaagatct gatcatatgc
      241 atccgcgggc ccgggttacg agatcgatga attcgagctc ggtaccccta gagtcgacct
      301 gcaggcatgc aagcttctcc ctatagtgag tcgtattagc ggccgcatcg aatataactt
      361 cgtataatgt atgctatacg aagttattag cgatgaggac atgaggttgc cccgtattca
      421 gtgtcgctga tttgtattgt ctgaagttgt ttttacgtta agttgatgca gatcaattaa
      481 tacgatacct gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt
      541 gatatgtaga tgataatcat tatcacttta cgggtccttt ccggtgatcc gacaggttac
      601 ggggcggcga cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc
      661 gttcttcttc gtcataactt aatgttttta tttaaaatac cctctgaaaa gaaaggaaac
      721 gacaggtgct gaaag
//
