LOCUS       sequence-130278-       11713 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB426
FEATURES             Location/Qualifiers
     source          1..11713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2766
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      2788..3040
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3074..3090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3074..3090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3087..3109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3098..3114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3122..3128,3129..3146,3147..3152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3167..3188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3273..3447
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3504..4301)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3615..3634)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4225..4244
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4364..5040)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4407..4427)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5235..5259
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5780..6571
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6817..7405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7306..7325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7559..7576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7591..7731
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7657..7676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7817..7839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8075..8269
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8335..9408)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9837..10466)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ctggaggctc cggtgggagc atgagcgagc tgattaagga gaacatgcac
     2101 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     2161 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     2221 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
     2281 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
     2341 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     2401 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2461 cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct
     2521 gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg gggccacctg
     2581 atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2641 ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
     2701 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     2761 aagtgagctt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     2821 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2881 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2941 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     3001 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     3061 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3181 tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta
     3241 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     3301 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     3361 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     3421 aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta gctagaggat
     3481 cgatccgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3541 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3601 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3661 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3721 aagcaggcat cgccatgtgt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     3781 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3841 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3901 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3961 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     4021 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     4081 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac
     4141 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4201 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4261 gccggagaac ctgcgtgcaa tccatcttgt tcaatcccca tggtcgatcg acagatctgc
     4321 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
     4381 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
     4441 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
     4501 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     4561 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     4621 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     4681 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     4741 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc
     4801 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     4861 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     4921 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     4981 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     5041 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     5101 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca gaaataaatt
     5161 cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5221 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5281 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5341 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5401 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5461 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5521 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     5581 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5641 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5701 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5761 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5821 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5881 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     5941 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     6001 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     6061 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     6121 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     6181 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6241 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6301 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6361 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6421 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6481 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6541 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6601 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6661 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6721 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6961 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7381 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7441 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7501 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7561 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7621 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7681 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7861 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7921 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7981 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     8041 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     8101 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     8161 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     8221 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8281 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8341 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8401 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8461 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8521 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     8581 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8641 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8701 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8761 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8821 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8881 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     8941 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     9001 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     9061 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     9121 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     9181 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9241 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9301 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9361 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     9421 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9481 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9541 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9601 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9661 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9721 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9781 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9841 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9901 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9961 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    10021 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    10081 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    10141 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    10201 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    10261 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    10321 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    10381 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    10441 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10501 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10561 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10621 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10681 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10741 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10801 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10861 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10921 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10981 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    11041 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    11101 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    11161 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    11221 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    11281 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    11341 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    11401 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    11461 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11521 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    11581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11641 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    11701 aacggataaa cct
//
