LOCUS       sequence-13030-e        4125 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1136-HQ858680
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1682..1781)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1798..1817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1799..1817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1822..1838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1822..1838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1940..1959)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1951..2760
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2018..2037)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2907..3495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3396..3415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3649..3666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3825..3852)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3944..4030)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4079..4101
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4093..4110
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4094..4110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggagctcg ccggagcagc ctcccctcac ccgccgccgg aatcccacgt ggcgccgccc
      181 cgcccacctc cgcagccgcc ggaagaggat ttatgtgaag atagagggga catgagtgtc
      241 accggcgaaa agccatgcac acatcgggaa tcagatgttg gtcagacaaa tagttgtagc
      301 cttaacaatt ccagtgagtg tgagaatcat acacccagca acgacgaaat atcggaacca
      361 gagtccaatt tggagatggc caagactgat cagggcggcg atgtgccgag cggagagaag
      421 gtcctgaaga agccagacaa gatcctgccg tgccctcgct gcaacagcat ggacacgaag
      481 ttctgctact acaacaacta caacatcaag cagccaaggc atttctgcaa gagctgtcag
      541 aggtactgga ccgcaggcgg aagcatgaga aacatccccg ttggtgctgg gaggcgcaag
      601 agcaagagct ccagcgcgag ttgccgcagc gtgttgattc ccgttccagg cagcagtagt
      661 gtagctaatc ctggcggaga ggcttccctg tttccgttgt ccgtaaaagc aaaccaagca
      721 gcagttagct tcgggcctga ttcccccctc tgcacctcca tggcctccgt gctgaagatc
      781 ggaggaggag gagagcaggt taagagctgc agccctgcct cagcagcagc acagcccagg
      841 aacggagaaa cccagaccca gacgtgcgca ccttcttctg ctgctacaac accatcagat
      901 ggtccaggga atggattgca gaaaggagca gagagcgcac accaaaacca aaaccaaaac
      961 caaaacggaa tcattgggca cagcaacgga gtcactccag tgcatcctat accgttcttc
     1021 cccggaccgc ctttcgtgta cccctggagt ccagcatgga acggcattcc cgccatggca
     1081 gcggcggtgt gcgcagcccc agcgacagcc gaagcagcga tttcatcaga acacggcacc
     1141 gcgagcagca ccgtccagtg gaacgtgcca ccagcgatcg tgcccgtgct gccaccggga
     1201 ttctgcggcc cgatcccagt cccggtaatc ccgccgcctt ccgtctggcc actgatcact
     1261 ccctggccca acgcagcatg gagcgcgccg tggctcgggc ctagcgctag cgtgccgccg
     1321 gggtcatctc ggagcagcgg cagcagcacg tgctccgaca gcggctgcgg ctccggctcc
     1381 cccgtcctgg gaaagcactc gagggagtcg aggccgcagg gcgacgagaa ggcggaacga
     1441 cggtgcctgt ggatccccaa gacgctccgg atcgacgacc ctgtcgaggc cgccaagagc
     1501 tcgatctgga cgacgctcgg gatcgagcct ggcgaccggg gcatgttcag gccgttccag
     1561 tcgaagcatg gacggcagca ggagctgcaa gcgtccggcg ccgctcgcgc cctgcaggcc
     1621 aacccggcgg ctctgtcgcg ctcgcagtct tttcaggaga cgacgaaggg tgggcgcgcc
     1681 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1741 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1801 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1861 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1921 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1981 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2041 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2101 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2161 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2221 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2281 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2341 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2401 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2461 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2521 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2581 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2641 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2701 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2761 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2821 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     3301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3661 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3721 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3781 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3841 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3901 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3961 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4021 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4081 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
