LOCUS       sequence-130301-       13724 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB108
FEATURES             Location/Qualifiers
     source          1..13724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3805..3807,3808..3810,3811..4521)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3850..3871)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4111..4130)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4458..4479
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4584..4836
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4870..4886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4870..4886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4883..4905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4894..4910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4918..4924,4925..4942,4943..4948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4963..4984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5069..5243
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5284..6309)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6376..7051)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7246..7270
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7791..8582
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8828..9416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9317..9336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9570..9587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9602..9742
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9668..9687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9828..9850
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10086..10280
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10346..11419)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11848..12477)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgctggag gctccggtgg gagcatggtg agcaagggcg aggagctgtt caccggggtg
     3841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3961 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc
     4021 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4201 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4261 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4321 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4381 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     4441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4501 ggcatggacg agctgtacaa gtaagcttgc atgcctgcag gtcgactcta gaggatcccc
     4561 gggtaccgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4621 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4681 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4741 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4801 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
     4861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4981 attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg tttttaatgt
     5041 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     5101 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     5161 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     5221 actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg gtcggcatct
     5281 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
     5341 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
     5401 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
     5461 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
     5521 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
     5581 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
     5641 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
     5701 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
     5761 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
     5821 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
     5881 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
     5941 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
     6001 caggtcttgc aacgtgacac cctgtgaacg gcgggagatg caataggtca ggctctcgct
     6061 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
     6121 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
     6181 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
     6241 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
     6301 ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata gatttgtaga
     6361 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
     6421 aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     6481 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     6541 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     6601 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     6661 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     6721 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     6781 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     6841 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     6901 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     6961 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     7021 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     7081 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     7141 ccaatacgca aaccgaattc agaaataaat tcagcctaat tcggcgttaa ttcagtacat
     7201 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     7261 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     7321 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     7381 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     7441 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     7501 tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     7561 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     7621 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     7681 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     7741 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
     7801 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7861 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7921 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     7981 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     8041 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     8101 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     8161 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     8221 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     8281 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     8341 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     8401 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     8461 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     8521 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     8581 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     8641 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     8701 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca
     8761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8941 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9601 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     9661 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     9721 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     9781 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9841 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     9901 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     9961 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
    10021 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
    10081 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
    10141 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
    10201 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
    10261 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
    10321 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
    10381 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
    10441 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
    10501 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
    10561 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
    10621 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
    10681 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
    10741 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
    10801 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
    10861 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
    10921 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
    10981 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
    11041 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
    11101 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
    11161 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
    11221 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
    11281 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
    11341 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
    11401 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    11461 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    11521 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    11581 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    11641 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    11701 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    11761 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    11821 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    11881 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    11941 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    12001 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    12061 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    12121 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    12181 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    12241 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    12301 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    12361 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    12421 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    12481 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    12541 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    12601 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    12661 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    12721 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    12781 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    12841 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    12901 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    12961 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    13021 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    13081 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    13141 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    13201 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    13261 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    13321 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    13381 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    13441 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    13501 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    13561 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    13621 agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac
    13681 cctgtggttg gcatgcacat acaaatggac gaacggataa acct
//
