LOCUS       sequence-130664-         737 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Optogenetic Ras activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Toettcher JE, Weiner OD, Lim WA
  TITLE     Using optogenetics to interrogate the dynamic control of signal 
            transmission by the ras/erk module.
  JOURNAL   Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
  PUBMED    24315106
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1422-34"
COMMENT     Sequence Label: pHR SFFVp YFP-PIF-SOS2cat
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(103..105,106..108,109..621)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(148..169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(409..428)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
       61 caagtggagc aaggcaggtg gacagtggat ccgcttggta ccatggtgag caagggcgag
      121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      181 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
      301 tacggcctga agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      541 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      601 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      661 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
      721 aagctgagca aagaccc
//
