LOCUS       sequence-130814-         923 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1C02
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(532..631)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(648..667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(649..667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(672..688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(790..809)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(868..887)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gcttcatgtc ggactgggac tctgtgactg
      121 ttcttcggaa gaaagcaccg aaatcgtcga ccctcaagac ggaatcggcg gtcaaccagg
      181 cacgccgtca aggagtcgcc gtggataccc aacagaagta tggtgttggc accaacaagc
      241 agcatgtgac caccaagaac actgccaaac tagaccgcga gaccgaggag ctgcgccacg
      301 acaagattcc cctcgatgtg ggcaagctca tccagcaggg acgacagagc aagggcctca
      361 gccaaaagga cctggcaact aaaatctgcg aaaagcagca agtggtaacc gactacgagg
      421 ccggtcgcgg tattccaaac aacttgattc tgggtaaaat ggaacgcgtt ctcggcatta
      481 agctgcgcgg caaggagcgc ggccaaccaa tagcacctcc cggtaaaaag gacccagctt
      541 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
      601 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
      661 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
      721 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      781 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
      841 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      901 caggtgcgac aatctatcgc ttg
//
