LOCUS       sequence-131003-         830 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCherry2-N1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..96
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..814
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(249..267)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      121 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      241 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      301 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      361 ctacttgaag ctgtccttcc ccgagggctt caattgggag cgcgtgatga acttcgagga
      421 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcagt gtcgtaccat
      541 gggctgggag gcctccactg agcggatgta ccccgaggac ggcgccctga agggcgagat
      601 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      661 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcgacatca agttggacat
      721 cctttcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      781 ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
//
