LOCUS       sequence-13103-e        4146 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1453-HQ858747
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1703..1802)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1819..1838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1820..1838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1843..1859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1843..1859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1961..1980)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1972..2781
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2039..2058)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2928..3516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3417..3436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3670..3687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3846..3873)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3965..4051)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4100..4122
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4114..4131
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4115..4131
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcgggt ggcggcgagg gggatcgcgt gggtgtcggg
      181 ggagcccagc agttcgtgga ccggagcaag gtgaggattc ttctctgcga cggcgacgcc
      241 accagctccc gggaggtgct gcggctcctc tgcaactgct cataccatgt gacctgcgcc
      301 aagtctccgc ggcaggtgat caatatcctc aactacgagg gcggcgagat cgacatcatc
      361 ctggctgagg tcgatcttcc ggtctccaag tgcttcaaga tgctcaagta cattgcccgg
      421 aacaaggacc tgcgtcacat ccccatcata atgatgtcca acagagacga ggtgtctgtt
      481 gttgtcaagt gcttgcggct tggcgccgcc gagtacctgg tgaagccgct gcgcacgaat
      541 gagttgctga acctttggac ccatgtgtgg cggcggagac ggatgcttgg tttgcccgag
      601 aaaaacttct tcaatgacaa cttcgagttg gtgctctcag aacctagcga tgccaatacc
      661 aatagcacca ccctcctctc agacgagacc gacgataggc ctaaaggaaa cacgaatcaa
      721 gaaacaggca cctcaaagca acttgaatac gagtctaatc cttcggttgc tgagcccgac
      781 cagagagaaa agatggaggg tgttccaggc tctgccctag atgccagtaa aaaatcctct
      841 ccaagaagag cgttttcacg ccctataaaa actaacctga gggttgccga gtcgtctgca
      901 tttctagcat atgtcaagcc aagcacccca gccaccagct catttgacag cgaattacaa
      961 aggggtggca gccgattaga ttccttggat aaccagggta attgctctag cgcaaccgat
     1021 agaagtgaca ctggtactga tgtaaatatt cggagtaaag aagcttttga gatgcctgcg
     1081 cagtacccta tggtatggtt ttcttcctct aacatgcata tggagcgaag cagcgaaggc
     1141 cataatgata cttcaggaac tccacctgca taccatttcc cattttatta cccagggatg
     1201 gtagagcaca acatggcact ttcgtcggcg caagattttc aagcaaacat aaacaatgct
     1261 caagcacata caccgccagc gatgcttcct cagtacaatg tttatcctca gtgccatggt
     1321 ttacctgtga taccatcatt tcagtttaat ccttctggca tgagtacgca ctcaagtcat
     1381 ttaccaacgc aaaatgtgtg gtcatcggca tcgagcacac caccagtgcc tgaggaaaca
     1441 tgcagtcgct ctgaaaggag ggctgcagca cttgccaagt tcaggctgaa gaggaaagag
     1501 cgctgctttg acaagaaggt gaggtatgtg aaccggaaga aactcgctga aacgaggcca
     1561 agggtgcgag gtcagtttgt tagacagcca accaatacgg atgtcatcag cattggagat
     1621 gatatatctg aagacgaaga tgacgatcca tcgtccaggg aggtagatat ggtttcttct
     1681 ccagagaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
     1741 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1801 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1861 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1921 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1981 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2041 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2101 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2161 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2221 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2281 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2341 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2401 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2461 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2521 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2581 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2641 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2701 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2761 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2821 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2881 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2941 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3001 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3061 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3121 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3181 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3241 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3301 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3361 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3421 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3481 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3541 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3601 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3661 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3721 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3781 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3841 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3901 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3961 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4021 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4081 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4141 gggccc
//
