LOCUS       sequence-13104-e        4350 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1454-HQ858748
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1907..2006)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2023..2042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2024..2042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2047..2063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2047..2063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2165..2184)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2176..2985
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2243..2262)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3874..3891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4050..4077)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4169..4255)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4304..4326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4318..4335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4319..4335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggccgtc cctcctctgc tgctcgtcgc cgttgccgta
      181 gccgcgctct ccatcctcgt cctcgtcgtc ttcgccttcc ggcgatgcag gcggcggcgg
      241 caacgccacc cgctgcaggc cgcggcggtg gccccgaccc cggtcgctgt ccaggatgag
      301 gacatagata ggcctcttct ttctgaaaat cagagcgatc actctagaca gaatattagt
      361 tttcttggaa gttctgtagg agaaccttcg aagattcaaa caaataggag taacacttcg
      421 cctagaagtc atgcaattgc tgatacaggg aggatttatc ctgctgaatg ttgtgttaca
      481 caaggagaaa ctcatgtaat caatgttgaa aatgatactt ctgaagagtt ccaattagga
      541 agcacactaa gacgtacacc gccaccaaaa tggccaacac ctgaccagaa gcacagaaga
      601 agggtttctg gagatgacaa tcataatgga agtgttcctt tgaaggataa tacatatcat
      661 agccgcctgg atttagaggt tatagctggt ccatctcatg gaataagctg ttctcggcag
      721 tcaagtagac cttctatgct cccaataact cttggaaggg ttcccccaag tgatttagtg
      781 ttgaaggact ctgaagtatc agggaaacat gctcagataa actggaatgg aaagacatta
      841 aaatgggaac ttgtggacat gggcagcttg aatggaactt tcttgaactc ccaggcggtt
      901 caccatccaa gtgctgggtc taggcattgg ggtgaacctg ctgaacttgc acatggtgat
      961 attataactc tagggacttc atctaaactg tctgttcaaa ttttactgca aaatcaacga
     1021 gttcctgctg gtgttggtat ggcatccgat ccaatggtgg cacgtagaag tggaaagaaa
     1081 cttccaatgg aagacataag cttttgccag tgtcctctgc agggtgttga acattttggg
     1141 ctctttggaa tttttgatgg acacggtggg gatggagctg ctaaagctgt cagcaagatt
     1201 cttccagaac atctgggata cattttatct catcctgaaa caaaagaacg agtgcaatca
     1261 tattctgatg cttctgatgt tcttagatat gcttttactt tgacagaaga cacaattgat
     1321 catcagtacg agggatgtac aggcacagca cttcttattt ggtttgacca gaataaagat
     1381 tgttttgccc agtgcgctaa tcttggtgac tctgcatgcg tcatgagtgt caacggcaag
     1441 acgatcgata tgactgaaga tcaccgggta actagcgcga ccgaaagagc taggatagca
     1501 agaactgggc agcccctgag agacggtgaa gctcgtctta gcggactaaa tcttgctagg
     1561 atgtttggtg acaagtttct caaggagcag gacccgcgct tcagctcaga accatacgtg
     1621 agccaagccg ttcgcatcac caaagcgtgc acagccttcg ccgtcattgc tagcgacggg
     1681 ctctgggacg tcatcagcac gaaaagggcg gtacagcttg tcgtcgaggg aaaggagagg
     1741 aggagctccg gcgacgccac ctcagcggcg gccagagtag ccagccgtgt gctggacgaa
     1801 gctcggagtc tgcgaaccaa ggacaacaca tccgtaatat tcgtagattt tgacgtcctg
     1861 agaagggatc cctgtatctc gccagttgat aagggtgggc gcgccgaccc agctttcttg
     1921 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1981 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2041 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2101 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2161 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2221 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2281 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2341 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2401 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2461 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2521 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2581 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2641 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2701 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2761 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2821 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2881 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2941 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3001 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3061 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3961 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4021 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4081 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4141 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4201 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4261 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4321 aaaacgacgg ccagtcttaa gctcgggccc
//
