LOCUS       sequence-131094-         822 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 
            2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: 2PH-PLCd-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attcaaggat caacaagttt gtacaaaaaa gcaggctgcc gccaccatgg actcgggccg
       61 ggacttcctg accctgcacg gcctacagga tgatgaggat ctacaggcgc tgctgaaggg
      121 cagccagctc ctgaaggtga agtccagctc atggaggaga gagcgcttct acaagttgca
      181 ggaggactgc aagaccatct ggcaggagtc ccgcaaggtc atgcggaccc cggagtccca
      241 gctgttctcc atcgaggaca ttcaggaggt gcgaatgggg caccgcacgg agggtctgga
      301 gaagttcgcc cgtgatgtgc ccgaggaccg ctgcttctcc attgtcttca aggaccagcg
      361 caatacacta gacctcatcg ccccatcgcc agctgatgcc cagcactggg tgctggggct
      421 gcacaaaatt atccatcaca gcggatctat ggaccagcgt cagaagctac agcactggat
      481 tcactcctgc ttgcgaaaag ctgacaaaaa caaggacaac aagataggcg gcagcggcgg
      541 cagcggcggc gatagcggta gagattttct gactctgcat ggactgcagg acgacgagga
      601 cctgcaggcc ctgctgaagg gcagccagct gctgaaggtg aagagcagca gctggcgccg
      661 cgagcgcttc tacaagctgc aggaggactg caagaccatc tggcaggaga gccgcaaggt
      721 gatgcgcacc cccgagagcc agctgttcag catcgaggac atccaggagg tgcgcatggg
      781 ccaccgcacc gagggcctgg agaagttcgc ccgcgacgtg cc
//
