LOCUS       sequence-131155-        9806 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses the extracellular region of the Neogenin 1, isoform g 
            protein, C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Neo1.g-AP-His
FEATURES             Location/Qualifiers
     source          1..9806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6386..6403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6429..6550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6466..6485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6520..6539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6588..6605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6759..7347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6839..6858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7518..8309,8310..8378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8141..8160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8379..8483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8551..8569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8607..8629)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8631..8651)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8636..8966
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8817..8952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8879..8898
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9103..9122
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9189..9644)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9326..9347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9538..9557
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9770..9792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9784..9801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9785..9801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcggcgga
     1621 gcgcgaagcc gggcgactcc tctgcacctc ctcctcccgg cgctgctgtc cgccaccgcc
     1681 gctgctgctg ttgctgccgc tgctgctgct gctcggacgc ccggcgtccg gcgccgcggc
     1741 cacgaagagc ggctccccgc cgcagtccgc aggagccagt gttcgaacat tcactccgtt
     1801 ttattttctg gtggagccag tagacaccct ctcagttaga ggctcttctg ttatattaaa
     1861 ttgctcggca tattctgagc cctctccaaa cattgaatgg aagaaagatg ggactttttt
     1921 aaacttagaa tcagatgatc gacgccagct actcccagat ggatctttat tcatcagcaa
     1981 cgtggtgcat tccaaacaca ataagcctga cgaaggtttc tatcagtgtg tagccactgt
     2041 ggataatctt ggaaccattg tcagcagaac agccaagctc acagtagcag gtcttccaag
     2101 atttaccagc caaccagaac cttcttcagt ctatgttgga aacagtgcaa ttctgaattg
     2161 tgaagttaat gcagatttgg tcccatttgt taggtgggaa cagaatcgac agccccttct
     2221 tctagatgac aggattgtca aacttccaag tggaacactg gttatcagca atgctactga
     2281 aggagatggg ggactctacc gctgcattgt tgaaagtggt gggccaccaa agtttagtga
     2341 cgaagctgaa ttgaaagttc ttcaagatcc tgaggaaatt gtagacttgg tatttctgat
     2401 gcgaccatct tctatgatga aagtcactgg tcagagtgca gtgttgccat gtgttgtctc
     2461 agggcttcct gctccagttg ttagatggat gaaaaacgaa gaagtgcttg acacagaaag
     2521 ctctggcagg ttggtcttgc tagcaggagg ttgcttggag atcagtgatg tcactgagga
     2581 tgatgctggg acttattttt gcatagctga taatggaaat aagacagttg aagctcaggc
     2641 ggagcttact gtgcaagtgc cacctggatt cctgaaacaa cctgctaaca tatatgctca
     2701 cgaatccatg gacattgtat ttgaatgtga agtcactggg aagccaactc caactgtgaa
     2761 gtgggtcaag aatggggatg tggttatccc cagtgattac tttaaaattg taaaggaaca
     2821 taatcttcaa gttttgggtc tggtgaaatc agatgaaggg ttctatcaat gcattgctga
     2881 gaatgatgtt ggaaatgcac aagctggagc ccagctgata atccttgagc atgcaccagc
     2941 cacaacggga ccattacctt cagctcctcg agacgtcgtg gcctccctgg tctctactcg
     3001 cttcattaaa ttgacatggc gtacacctgc atcagaccct catggagaca atctcaccta
     3061 ctctgtgttc tacaccaagg aaggggttgc tagggagcgt gttgagaata ccagccagcc
     3121 aggagagatg caggtgacta ttcaaaactt gatgccagca actgtgtaca tcttcaaagt
     3181 tatggctcaa aataagcatg gctctggaga aagttcagct cctcttcgag tagagacaca
     3241 gcctgaggtt cagctccctg gcccagcacc taatatccgt gcttatgcaa cgtcacctac
     3301 ttctatcact gtcacctggg aaacaccgtt atctggcaat ggggaaattc aaaattacaa
     3361 attgtactac atggaaaaag gaactgataa agaacaggat attgatgttt caagtcactc
     3421 ctacaccatt aatggactga agaaatacac agaatacagt ttccgagtgg tggcctacaa
     3481 taaacatggt cctggagttt ctacacaaga tgttgctgtt cgaacattat cagatgttcc
     3541 cagtgctgct cctcagaatc tgtccttaga agtgagaaat tcaaagagta tagtgatcca
     3601 ctggcagccc ccttcctcaa ccacacaaaa tgggcagata actggctaca agattcgata
     3661 tcgaaaggcc tcccgaaaaa gtgatgtcac tgagaccttg gtaactggga cacagctgtc
     3721 tcagctgatt gaaggtcttg atcgggggac agaatataac ttccgagtcg ctgctctcac
     3781 agtcaatggt acaggtccag caactgattg gctgtctgct gaaacttttg aaagcgacct
     3841 agatgaaact cgtgttcctg aagtgcccag ctctcttcat gtccgtccgc tcgtcactag
     3901 cattgtagtg agctggactc ctccagagaa ccagaacatt gtggtccgag gttatgccat
     3961 cggttacggc attggcagcc ctcatgccca gaccatcaaa gtggactata aacaacgtta
     4021 ttacaccatc gaaaacttgg atccaagctc tcattacgtg attaccttga aagcatttaa
     4081 caatgttggc gaaggcatcc ccctttatga gagtgctgtg accagacctc acacagtgcc
     4141 agatcccact cccatgatgc caccagtggg agttcaggct tccattctga gtcacgacac
     4201 cataaggatt acctgggcag acaactccct gcccaaacac cagaagatta cagactcccg
     4261 ctactacaca gtccggtgga agaccaacat cccagcaaac acgaagtaca agaatgcaaa
     4321 tgcaacgacg ttaagctatt tggttactgg tttaaagcca aatacgctct atgagttctc
     4381 tgtgatggtg accaaaggca gaaggtcaag cacgtggagt atgacagctc atggcgctac
     4441 ctttgaatta gttcctactt ctccacctaa ggatgtgaca gttgtgagta aggaaggaaa
     4501 acctagaacc atcatagtga actggcagcc tccctctgaa gctaacggca agattacagg
     4561 ttacatcatc tattacagca cggatgtgaa tgcagagata catgactggg ttattgaacc
     4621 agttgtggga aacagactga ctcaccagat tcaagagtta acacttgata cgccatacta
     4681 cttcaaaatc caggcccgga actcaaaggg catggggccc atgtctgaag ctgtacagtt
     4741 cagaacacct aaagcggact cctctgataa aatgcctaat gaccaagcct tagggtcagc
     4801 aggaaaagga agccgactac cagacctggg atctgactac aaacctccaa tgagtggcag
     4861 caacagccct cacgggagcc ccacctcccc tctggacagc actagtggat cttccggaat
     4921 catcccagtt gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg
     4981 tgccgccaag aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg
     5041 cgatgggatg ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga
     5101 caaactgggg cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa
     5161 gacatacaat gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg
     5221 cggggtcaag ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg
     5281 caacacgaca cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa
     5341 gtcagtggga gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc
     5401 ccacacggtg aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga
     5461 ggggtgccag gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg
     5521 tggaggccga aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta
     5581 cagccaaggt gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg
     5641 ccagggtgcc cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc
     5701 tgtgacccat ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga
     5761 ctccacactg gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag
     5821 gaacccccgc ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga
     5881 aagcagggct taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc
     5941 gggccagctc accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt
     6001 cttctccttc ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa
     6061 ggcccgggac aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct
     6121 caaggacggc gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca
     6181 gcagtcagca gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc
     6241 gcgcggcccg caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt
     6301 catggccttc gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg
     6361 caccaccgac gccgcgcacc cgggtcatca tcaccatcac cattaatcta gagatcttat
     6421 taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6541 atgtatctta tcatgtctgg tcgactctag ctagactctt ccgcttcctc gctcactgac
     6601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8521 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     8581 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga ggaatgtgtg
     8641 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     8701 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8761 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8821 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8881 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     8941 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcgtcc
     9001 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9061 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     9121 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     9181 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9241 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9301 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9361 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9421 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9481 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9541 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9601 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     9661 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9721 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9781 cgttgtaaaa cgacggccag tgaatt
//
