LOCUS       sequence-131269-        9086 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the extracellular region of the L1CAM protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: L1cam-Fc-His
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5666..5683
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5709..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5746..5765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5800..5819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5868..5885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6039..6627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6119..6138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6798..7589,7590..7658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7421..7440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7659..7763)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7831..7849
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7887..7909)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7911..7931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7916..8246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8097..8232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8159..8178
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8383..8402
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8469..8924)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8606..8627)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8818..8837
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9050..9072
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9064..9081
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9065..9081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggtcgtgat
     1621 gctgcggtac gtgtggcctc tcctcctctg cagcccctgc ctgctcatac agattccaga
     1681 cgaatataaa ggacaccatg tgctagagcc acctgtcatc acggaacagt ctccacggcg
     1741 cctggttgtc ttcccaacag atgacataag cctgaaatgt gaagccagag gcagacccca
     1801 agtggagttc cgctggacga aagatggcat ccacttcaaa cccaaggaag aattgggtgt
     1861 agtggtgcat gaggcaccct attctggctc cttcaccatc gaaggcaaca acagctttgc
     1921 ccagaggttt cagggcatct atcgctgcta tgccagcaat aagctaggaa ctgccatgtc
     1981 gcatgagatc cagctcgtgg ccgagggtgc ccccaagtgg ccgaaggaga ctgtaaaacc
     2041 tgtggaagtg gaggaaggag aatcagtagt tctgccttgc aaccctccac ccagtgcagc
     2101 cccacttagg atctactgga tgaacagcaa gattttgcac atcaaacaag atgagcgggt
     2161 gtccatgggc cagaatggag acctatattt tgccaatgtg cttacctcag acaatcattc
     2221 agactacatc tgcaatgccc acttccctgg tacccggacc atcattcaaa aggaacctat
     2281 tgacctccgg gtcaagccca ccaacagcat gattgaccgg aagccacgtc tgctctttcc
     2341 cacaaactcc agcagccgcc tggtagcctt gcagggccag tcattgatcc tggagtgcat
     2401 tgctgaggga ttccctacac ccaccatcaa gtggctgcac cccagtgacc caatgccaac
     2461 agaccgtgtt atctaccaaa accacaacaa gaccctgcaa ctactcaatg tgggcgaaga
     2521 ggacgatggc gagtatacct gccttgctga gaactcgctg ggcagtgccc ggcatgccta
     2581 ctatgttact gtggaagctg ccccatattg gctgcagaag ccccagagcc atttgtatgg
     2641 tccaggagag actgcccgcc tagactgcca agtccagggc aggccccaac cagagatcac
     2701 ttggagaatc aacggaatgt ctatggagac ggtgaacaag gaccagaagt accggattga
     2761 gcaggggtct ctgatcttga gtaacgtgca gccaagtgac acaatggtga cccagtgtga
     2821 agcccgcaac cagcatgggc tcctgctagc caatgcctac atttatgttg tccagctgcc
     2881 agccaggatc ctaacaaaag acaatcagac atacatggca gttgagggca gtactgctta
     2941 cttgctgtgc aaagcctttg gagctcctgt tcccagtgtc cagtggctgg atgaagaagg
     3001 aaccacagtg cttcaggatg aacgattttt cccctatgcc aatggaacgc tgagcatcag
     3061 agacctccag gccaatgaca ctggacgcta tttctgccag gctgccaatg accagaacaa
     3121 tgtgaccatt ttggctaacc tacaggttaa agaagcaacc cagatcacac aggggccccg
     3181 gagcgcaatt gagaagaaag gtgcaagggt gacattcacg tgccaggcct cctttgaccc
     3241 ctctttgcag gccagcatca cttggcgtgg agatgggaga gacctacagg aacgtgggga
     3301 cagtgacaag tatttcatag aagatgggaa actagtcatc cagagcctgg actacagtga
     3361 ccagggcaac tacagttgtg tggccagcac tgaactggat gaggtggaga gcagggcaca
     3421 gctcttagtg gtggggagcc ctgggccagt gcctcacctg gagctgtccg accgccacct
     3481 gctgaagcag agccaggtgc acttgtcttg gagccctgct gaagaccaca actctcccat
     3541 tgagaagtat gacattgaat ttgaggacaa ggaaatggct cctgagaaat ggttcagtct
     3601 gggcaaggtg ccaggaaatc agacctctac taccctcaag ctgtccccct atgtccacta
     3661 cacctttcgg gtcactgcca ttaacaaata tggtcctgga gaacccagcc ctgtctctga
     3721 gactgtggtc acacctgagg cagccccaga gaagaaccct gtggatgtga gaggggaagg
     3781 gaatgagacc aacaatatgg tcatcacatg gaagcccctt cggtggatgg attggaatgc
     3841 cccccagatt cagtaccgtg tacagtggcg tccacagggc aagcaggaga cctggaggga
     3901 acagaccgtg agcgaccctt tcctggtggt gtctaacact tccacatttg tgccttatga
     3961 gatcaaagtc caggcagtga acaaccaggg caagggccct gagccccagg tcaccattgg
     4021 ctattcaggg gaagactacc cccaggtgag ccctgaactt gaagacatca caatcttcaa
     4081 ctcaagtact gtgcttgtca ggtggaggcc tgtggacttg gcccaggtta agggccacct
     4141 caagggatac aatgtaacat actggtggaa gggcagccag agaaagcaca gcaagaggca
     4201 tatccacaaa agccacatag tggtacctgc aaataccacc agtgccatcc tcagtggttt
     4261 gcgcccttac agctcttacc atgtggaggt acaggccttt aatgggcggg gcttggggcc
     4321 tgcgagtgaa tggaccttca gcaccccaga gggagtgcct ggccaccctg aggcattaca
     4381 cctggagtgt cagtcggaca ctagtctgct actgcactgg cagccaccac tcagccacaa
     4441 tggagtgctc actggctacc tgctctctta ccatcccgtg gaaggggaaa gcaaagagca
     4501 gttgttcttc aacctttcgg acccagaact ccggactcat aatctgacca acctcaaccc
     4561 tgatctacag taccgcttcc agcttcaggc caccacccaa cagggtcctg gtgaggccat
     4621 cgtgcgtgaa ggaggcacca tggccctgtt tggcaagcca gattttggca acatctcagc
     4681 cacagcaggt gaaaactaca gcgtggtctc ctgggtccct cggaagggcc agtgcaattt
     4741 caggttccat atcttgttca aagccttacc agaagggaaa gtgagccctg atcaccagcc
     4801 tcagcctcag tatgtcagct acaatcagag ctcctacaca caatggaacc tacagcctga
     4861 caccaaatat gagatccacc tgataaagga gaaggtcctc ctgcaccatc tggatgtgaa
     4921 gactaatgga actggccctg tgcgagtttc tactacaggt agctttgcct cctctagtga
     4981 caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt
     5041 cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg
     5101 cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
     5161 cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
     5221 tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
     5281 caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg
     5341 gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa
     5401 ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg
     5461 ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
     5521 cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
     5581 cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacac agaagagcct
     5641 ctccctgtct ccgggtaaag gcggccatca tcaccatcac cattaatcta gagatcttat
     5701 taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5821 atgtatctta tcatgtctgg tcgactctag ctagactctt ccgcttcctc gctcactgac
     5881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7801 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     7861 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga ggaatgtgtg
     7921 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     7981 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8041 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8161 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     8221 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcgtcc
     8281 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8341 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     8401 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8461 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8521 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     8581 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     8641 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     8701 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     8761 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     8821 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     8881 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     8941 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9001 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9061 cgttgtaaaa cgacggccag tgaatt
//
