LOCUS       sequence-131497-         752 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-R3
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..59)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(86..712)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 ggcggggcgt aaacgccatg ggcatgtagt caaaagcctc cggtcggagg cttttgactt
       61 ggctgaggag tgccgttaat taagtttaaa ccgccggaga tttggtggtg tgcaggattt
      121 taacctggat catgatagac agttcagaac gacgtttttc cgcacgtttc agggtttttt
      181 ccagccaacg gttttcgttc agctggttgt gagaagactg gatgaagatt ttacggcagc
      241 actggtagtg ttcgctaacc atcacgttca gaccaacacc gttgtcacgc aggttccaca
      301 gaccgatctg gttacgcgcg tttttttcgt agtacagttt gcacgcccag attttcaggg
      361 tgtgaccgtt accacgcagt tcctggttgt accattccag gattttttcc gcgcagtccg
      421 cgcacggaga ccaagaagag taccagttga tggtgaactg acccgggttg tcacgcaggt
      481 attcttcaac tttacggata gagaagattt ccgcgtggat accacgttcg gtaccagact
      541 gcggtttgtt aaccgcgtaa ccccagaagc acgcacgacg ttcaccacga cgtttcagtt
      601 cgaacagaac gtagcaacgg tgagaaacag attttttgtt gttgaagaac tgttttttga
      661 aggtgtagat gtccagtttt tcgtggatac gaacgtattc cgcgtcggtc atcttcagat
      721 tccggtttac ttaatctcga cttacaacat tt
//
