LOCUS       sequence-131508-         970 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  An AAV packaging vector that expresses ER-retained GCaMP3 under 
            control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(149..157,158..172)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             200..256
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     469..490
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(596..617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(857..876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgtcgtgag gtaccggatc cgctagccac catgctgcta tccgtgccgt tgctgctcgg
       61 cctcctcggc ctggccgtcg ccatgggttc tcatcatcat catcatcatg gtatggctag
      121 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc
      181 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
      241 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
      301 caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
      361 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      421 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
      481 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
      541 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      601 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
      661 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      721 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      781 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
      841 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      901 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      961 cctggggcac
//
