LOCUS       sequence-131544-         752 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of Drosophila melanogaster Rpl10Ab-GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, 
            Chang P, Nuzhdin S, Arbeitman MN, Dierick HA
  TITLE     A versatile method for cell-specific profiling of translated mRNAs 
            in Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 
            2012 Jul 6.
  PUBMED    22792260
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7):e40276. doi"; pages: " 
            10.1371/journal.pone.0040276. Epub 2012 Jul 6"
COMMENT     Sequence Label: pUAST-GFP-RpL10Ab
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..752
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(69..97)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     692..712
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atagggaatt gggaattcgt taacagatct gcggccgcat gagtaaagga gaagaacttt
       61 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      121 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      181 gcactactgg aaaactacct gttccatggc caacacttgt cactacttta acttatggtg
      241 ttcaatgctt ttcaagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      301 tgcccgaagg ttatgtccag gaaagaacta tatttttcaa agatgacggg aactacaaga
      361 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      421 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      481 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      541 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      601 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      661 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      721 ggattacaca tggcatggat gaactataca aa
//
