LOCUS       sequence-13171-e        3861 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1232-HQ858815
FEATURES             Location/Qualifiers
     source          1..3861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1418..1517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1534..1553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1535..1553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1558..1574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1558..1574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1676..1695)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1687..2496
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1754..1773)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3385..3402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3561..3588)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3680..3766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3815..3837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3829..3846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3830..3846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggggaggc attcttgctg ctacaagcag aaattgagga aggggctctg gtcccctgag
      181 gaggatgaga agctcatgaa tcacataacc aagcatggcc atggctgctg gagttctgtt
      241 cctaaacttg cagggctcca aagatgcggc aagagctgca ggctgaggtg gataaactat
      301 ctgaggcctg acctcaagag aggtgcattc tctcaggagg aagaagacct catcattgag
      361 ctccatgctg tccttgggaa caggtggtct cagattgcag ctcagctgcc tgggaggact
      421 gacaacgaga tcaagaatct ctggaattct tgcatcaaga agaagctccg gcagaaaggc
      481 attgatccca acacccacaa gcccctcacc gaggctgatc gcaggggagc agcacccaca
      541 gtcagcaccg agaggacctc agggtccagc gacgtcaacc cgtcaagtgc tggtgctcta
      601 gggaacttga gccacctcct cagcgagaca gcacaatcat caatgctgct gccggtgtat
      661 gataagaatc accctgaaac tgcaagcttg ccacgcccta aggtgccacc aaaggagttg
      721 tttcttgacc agcttactgc cggtcacgag agcccatcaa gttgccgctc atcaggtcca
      781 accctgtatt ttcctttcca gcaaccatta ggctacagta atgaatgtgg cactggggat
      841 ggtgcgagta tgaattcact ctggttcaac cagaatgatt tcaactgcag cacaatctcc
      901 accgtgatgc caccagtttc accatcagcc ctctcaacat caatggggct gaatcttccc
      961 ccagaaaatc ctcgtcacgg aggcactggc attggcaaca ccccgttcta ctgggatggt
     1021 tccaatccta gcagcagcgg cagtactggg agcagcggaa gcaacagtat gggattcgag
     1081 ccacagagca caacctcaat tttggagaac agtgtattcc catggacaga tatcggacaa
     1141 gagaaagata ccagagttca cttagtggag gaactcaagt ggcctgattt gctccatgga
     1201 acctttgctg aggcgacgac agccatgcag aaccagagcc aatcattata cgatgatgtg
     1261 atcaaggcag agagccaatt caacatggaa gggatatgtg cctcttggtt ccagaatcag
     1321 caaccacaac aacagctgca agcagcatca gacatgtatg ataaagactt gcaaagattg
     1381 cccttgtctt ttgaacatat caagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1441 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1501 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1561 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1621 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1681 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1741 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1801 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1861 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1921 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1981 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2041 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2101 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2161 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2221 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2281 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2341 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2401 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2461 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2521 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2581 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3601 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3661 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3721 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3781 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3841 gccagtctta agctcgggcc c
//
