LOCUS       sequence-131756-         968 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  FLAG-Mnase expressed under the REB1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
  TITLE     ChEC-seq kinetics discriminates transcription factor binding sites 
            by DNA sequence and shape in vivo.
  JOURNAL   Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
  PUBMED    26490019
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-22"; volume: "6:8733. doi"; pages: " 
            10.1038/ncomms9733"
COMMENT     Sequence Label: pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             368..433
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             440..886
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     CDS             890..910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(936..952)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(936..952)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 attttacgta aactttcaat acattgaaat tgtgtctcat tttcttctag aagctcctcg
       61 gaaaaattga tcacgtgata tatagcaaca actttgttac ccgtctttca aattgggccc
      121 atgccgggtg gcaaaccaaa ggcggccaag ggacctaccc tcacaaagga caacgacaaa
      181 caaatggcag ctttcttgac cacacaagat aaaacaacaa aactcaggct gttattaccc
      241 tgctgtggac ccaacctaaa gtaaataaat aaacatcatc acaagcaagg ttatctacac
      301 actatcgtta ttagtttgaa ctcacgtgtt gctgctgaat tagaatattt atagctcaat
      361 caatatggat tacaaggatc acgatggcga ttacaaggat cacgatatcg attacaagga
      421 tgatgatgat aaggcggccg ccaccagtac caagaagctg cacaaggagc ccgccaccct
      481 gatcaaggcc atagatggcg ataccgtgaa gctgatgtat aagggccagc ccatgacctt
      541 ccgcctgctg ctggtggata cccccgagac caagcacccg aaaaagggcg tggaaaagta
      601 cggacccgag gccagcgcct tcacaaaaaa gatggtggag aacgccaaga aaatcgaggt
      661 ggagttcgat aaaggccaac gcaccgataa gtatggacgc ggtctggcct acatctacgc
      721 cgacggcaaa atggtgaacg aggccctggt gcggcaggga ctggccaagg tggcgtacgt
      781 gtacaagccc aacaacaccc acgagcagca cctgcgcaag agcgaggctc aggcaaagaa
      841 ggagaaactg aacatctgga gtgaggataa cgccgatagc ggccagccac caaaaaaaaa
      901 aagaaaagtt tagtaggaat tcctgcagcc cgggggatcc actagttcta gagcggccgc
      961 caccgcgg
//
