LOCUS       sequence-131832-         727 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses mutated p53-L344P tagged with mKate2 and split C-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(616..640)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccctcctca gcatcttatc cgagtggagg gaaatttgcg tgtggagtat ttggatgaca
       61 gaaacacttt tcgacatagt gtggtggtgc cctatgagcc gcctgaggtt ggctctgact
      121 gtaccaccat ccactacaac tacatgtgta acagttcctg catgggcggc atgaaccgga
      181 ggcccatcct caccatcatc acactggagg atagctctgg aaacctactg ggacggaaca
      241 gctttgaggt gcgtgtttgt gcctgtcctg ggagagaccg gcgcacagag gaagagaatc
      301 tccgcaagaa aggggagcct caccacgagc tgcccccagg gagcactaag cgagcactgc
      361 ccaacaacac cagctcctct ccccagccaa agaagaaacc actggatgga gaatatttca
      421 cccttcagat ccgtgggcgt gagcgcttcg agatgttccg agagccgaat gaggccttgg
      481 aactcaagga tgcccaggct gggaaggagc caggggggag cagggctcac tccagccacc
      541 tgaagtccaa aaagggtcag tctacctccc gccataaaaa actcatgttc aagacagaag
      601 ggcctgactc agacgaccca gctttcttgt acaaagtggc catggtgtct gagctgatta
      661 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      721 gcacatc
//
