LOCUS       sequence-13195-e        4146 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1506-HQ858839
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1703..1802)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1819..1838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1820..1838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1843..1859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1843..1859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1961..1980)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1972..2781
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2039..2058)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2928..3516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3417..3436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3670..3687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3846..3873)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3965..4051)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4100..4122
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4114..4131
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4115..4131
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggacagtg gcttgggaag aagttcagag acatccttga aagccttgcc atcaatggca
      181 agtaatgcaa caaggaatac tgatcctgac caacagggtg ttcgattcag ttccatggac
      241 cagcctccat gttttgcaag acctggtcaa tcgttccctg cttttcctcc actctttggg
      301 gttcagtctt ccagcttgta tttacctgat gacattgaag ctaaaatcgg taaccagttc
      361 gaatcaaatc cttccccgaa taatcctaca atggattggg accctcaggc aatgctgagc
      421 aacttatcct tccttgagca gaagatcaag caggtaaaag acatcgtgca gtctatgagt
      481 aatcgtgaga gccaagttgc tggtggttcc agcgaggcac aagcaaagca gcagcttgtc
      541 actgctgatc tcacttgtat tataattcag cttatttcaa cagctggttc cttgcttcct
      601 tcgatgaaga acccaatcag cagcaacccg gcactcagac atctcagtaa cacactttgt
      661 gctcctatga tcctgggcac caattgtaac ctgcgaccaa gcgcaaacga cgaagccaca
      721 attcctgaca ttagcaagac ccatgactat gaggagctga tgaatagcct taatactact
      781 caggcagaaa gcgatgagat gatgaactgc caaaatcctt gtggtgggga agggtcagaa
      841 ccgattccaa tggaagacca tgatgtgaag gagagtgatg atggtggtga gagagagaat
      901 ctcccccctg ggtcttatgt agtattgcaa ttagagaagg aggagatttt agcaccacat
      961 actcacttct gcttgatctg tggcaagggt tttaaaagag atgctaatct taggatgcac
     1021 atgaggggcc atggagacga gtacaaaact gctgcagctc ttgcgaaacc ttcgaaagat
     1081 tctagcttag agtctgcacc agttacaagg tactcgtgcc catatgttgg ctgcaagcgg
     1141 aacaaagagc acaagaagtt ccagcctctc aagacaatcc tgtgtgtgaa gaaccactac
     1201 aagagaagcc actgtgacaa gagctacacc tgcagccgtt gcaacaccaa gaagttctca
     1261 gttatcgcgg acttgaagac tcatgaaaag cactgtggcc gcgacaagtg gctatgctcg
     1321 tgtggaacta ccttctcaag aaaagacaag ttatttgggc atgttgcttt tttccaaggg
     1381 cacacgcctg cactccctat ggatgatatc aaagtaacag gagcatcaga gcaacctcaa
     1441 ggcagcgagg cgatgaacac catggtgggg agcgctgggt ataacttccc cggtagctca
     1501 tcggacgaca tcccaaatct tgacatgaag atggctgatg atccacgcta tttctcgcca
     1561 ttgagctttg atccttgctt cggtgggctt gatgacttca ctcgacctgg atttgacatc
     1621 tctgagaatc ccttctcctt cttgccctca ggatcatgca gcttcgggca gcagaacgga
     1681 gacagcaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
     1741 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1801 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1861 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1921 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1981 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2041 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2101 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2161 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2221 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2281 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2341 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2401 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2461 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2521 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2581 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2641 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2701 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2761 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2821 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2881 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2941 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3001 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3061 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3121 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3181 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3241 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3301 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3361 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3421 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3481 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3541 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3601 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3661 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3721 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3781 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3841 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3901 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3961 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4021 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4081 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4141 gggccc
//
