LOCUS       sequence-132186-         800 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  AAV2 transfer vector containing the InSynC 
            (SYP1-miniSOG-T2A-mCherry) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV-SYP1-miniSOG-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..178)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(206..523)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
       61 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatcg
      121 tacgagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctcac
      181 cgccaccaga tccacctccg ctagctccat ccagctgcac tccgatgaaa tactggagct
      241 cacccttctg gtccctcatg ggctggaggt gcaggaggtt ccagaatttc ttcccgcttt
      301 ttgtgtagtt tatcagctgc acggtaatct cgcgctgatc tctaatggca tcgcgaatct
      361 tctgcactgt ggcctggtct gtctctggtc cctgcagaaa gcggccattg cggcccagga
      421 tctcctctct ggaatactcg gtcagctcca ggaagccatc ggatgcgaag atgatgggat
      481 tgtctggcag ccgtggatca gttatcacga aacttttctc catgctgccg ccaccactgc
      541 cgccaccacc ggttttcatc tgattggaga aggaggtggg cgcaccctgt tggccatagc
      601 cttgctgccc atagtcgccc tgaggcccgt agccaccgcc accgctggct ggctgcccgt
      661 aatcgggttg ataaccaccc tgaggcccgt aggagtcctg gggcccatag cctccggggc
      721 cctgcccgta gcccgcatcg ccgtaggcat cgccaggtgc tggttgcttt tccggggcgc
      781 ctggaggtgc gcgcatgaat
//
