LOCUS       sequence-132265-        8902 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses diffuse K560-mCherry-eDHFR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Ballister ER, Ayloo S, Chenoweth DM, Lampson MA, Holzbaur EL
  TITLE     Optogenetic control of organelle transport using a photocaged 
            chemical inducer of dimerization.
  JOURNAL   Curr Biol. 2015 May 18;25(10):R407-8. doi: 
            10.1016/j.cub.2015.03.056.
  PUBMED    25989077
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2015.03"; journalName: "Curr Biol"; date: 
            "2015-05-18- 18"; volume: "25"; issue: "10"; pages: "R407-8"
COMMENT     Sequence Label: pERB258
FEATURES             Location/Qualifiers
     source          1..8902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             350..949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(350..369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     846..866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1085..1102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1091..1202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1347..1650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1652..1929
                     /label=chicken beta-actin promoter
     intron          1930..2946
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2870..2892
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2954..2973
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4711..5418
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4856..4874)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5124..5143
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5437..5910
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             5911..5940
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6047..6066)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6112..6167
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6166..6185)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    6530..6563
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6576..6697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6613..6632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6667..6686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6716..6736)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6716..6734)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6755..6771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6755..6771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6768..6790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6779..6795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6803..6809,6810..6827,6828..6833))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6848..6869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6986..7003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7157..7745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7237..7256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7916..8707,8708..8776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8539..8558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8777..8881)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgccat ggcggacctg gccgagtgca acatcaaagt gatgtgtcgc
     3061 ttcagacctc tcaacgagtc tgaagtgaac cgcggcgaca agtacatcgc caagtttcag
     3121 ggagaagaca cggtcgtgat cgcgtccaag ccttatgcat ttgatcgggt gttccagtca
     3181 agcacatctc aagagcaagt gtataatgac tgtgcaaaga agattgttaa agatgtactt
     3241 gaaggatata atggaacaat atttgcatat ggacaaacat cctctgggaa gacacacaca
     3301 atggagggta aacttcatga tccagaaggc atgggaatta ttccaagaat agtgcaagat
     3361 atttttaatt atatttactc catggatgaa aatttggaat ttcatattaa ggtttcatat
     3421 tttgaaatat atttggataa gataagggac ctgttagatg tttcaaagac caacctttca
     3481 gttcatgaag acaaaaaccg agttccctat gtaaaggggt gcacagagcg ttttgtatgt
     3541 agtccagatg aagttatgga taccatagat gaaggaaaat ccaacagaca tgtagcagtt
     3601 acaaatatga atgaacatag ctctaggagt cacagtatat ttcttattaa tgtcaaacaa
     3661 gagaacacac aaacggaaca aaagctgagt ggaaaacttt atctggttga tttagctggt
     3721 agtgaaaagg ttagtaaaac tggagctgaa ggtgctgtgc tggatgaagc taaaaacatc
     3781 aacaagtcac tttctgctct tggaaatgtt atttctgctt tggctgaggg tagtacatat
     3841 gttccatatc gagatagtaa aatgacaaga atccttcaag attcattagg tggcaactgt
     3901 agaaccacta ttgtaatttg ctgctctcca tcatcataca atgagtctga aacaaaatct
     3961 acactcttat ttggccaaag ggccaaaaca attaagaaca cagtttgtgt caatgtggag
     4021 ttaactgcag aacagtggaa aaagaagtat gaaaaagaaa aagaaaaaaa taagatcctg
     4081 cggaacacta ttcagtggct tgaaaatgag ctcaacagat ggcgtaatgg ggagacggtg
     4141 cctattgatg aacagtttga caaagagaaa gccaacttgg aagctttcac agtggataaa
     4201 gatattactc ttaccaatga taaaccagca accgcaattg gagttatagg aaattttact
     4261 gatgctgaaa gaagaaagtg tgaagaagaa attgctaaat tatacaaaca gcttgatgac
     4321 aaggatgaag aaattaacca gcaaagtcaa ctggtagaga aactgaagac gcaaatgttg
     4381 gatcaggagg agcttttggc atctaccaga agggatcaag acaatatgca agctgagctg
     4441 aatcgccttc aagcagaaaa tgatgcctct aaagaagaag tgaaagaagt tttacaggcc
     4501 ctagaagaac ttgctgtcaa ttatgatcag aagtctcagg aagttgaaga caaaactaag
     4561 gaatatgaat tgcttagtga tgaattgaat cagaaatcgg caactttagc gagtatagat
     4621 gctgagcttc agaaacttaa ggaaatgacc aaccaccaga aaaaacgagc agctgagatg
     4681 atggcatctt tactaaaagg accggtcacc atggtgagca agggcgagga ggataacatg
     4741 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4801 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4861 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4921 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4981 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     5041 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     5101 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     5161 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     5221 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     5281 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     5341 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5401 atggacgagc tgtataaggc cggcctggag tctagaatca gtctgattgc ggcgttagcg
     5461 gtagatcgcg ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc cgatctcgcc
     5521 tggtttaaac gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca
     5581 atcggtcgtc cgttgccagg acgcaaaaat attatcctca gcagtcaacc gggtacggac
     5641 gatcgcgtaa cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca
     5701 gaaatcatgg tgattggcgg cggtcgcgtt tatgaacagt tcttgccaaa agcgcaaaaa
     5761 ctgtatctga cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag
     5821 ccggatgact gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac
     5881 agctattgct ttgagattct ggagcggcgg gaacaaaaac tcatctcaga agaggatctt
     5941 taattaagcg ctcgaggcgg ccgcaattca ctcctcaggt gcaggctgcc tatcagaagg
     6001 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     6061 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     6121 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     6181 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     6241 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     6301 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6361 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6421 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6481 tcttatgaag atccctcgac ctgagcgtcg agctagcata tggatccata taacttcgta
     6541 taatgtatgc tatacgaagt tattacgata ccgtcaactt gtttattgca gcttataatg
     6601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt acccagcttt tgttcccttt
     6721 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8881 gcacatttcc ccgaaaagtg cc
//
