LOCUS       sequence-132274-         681 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  wild-type qacR downstream of a Tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES002 (pTet-qacR wt)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             173..184
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gcttccgatg gcgcgccgag aggctttaca ctttatgctt ccggctggtc tcagcatcca
       61 cgcattataa cgtcgtatta gctgcctttc gtcttcaata attcttgaca tccctatcag
      121 tgatagagat acttcgtgca atttttatat caccgccagg ggtacaacaa gcaaagagga
      181 gaaatactag catgaacttg aaagataaga ttttgggtgt tgctaaagag ctgtttatca
      241 aaaacggtta caatgctact accactggtg agattgtgaa attgtctgaa tcttctaaag
      301 ggaatctata ctatcatttc aaaacgaagg aaaatttgtt cttggaaatc ttgaacattg
      361 aagagagtaa atggcaagaa caatggaaaa aggagcagat taaagctaaa actaatagag
      421 aaaagtttta tctgtataat gagttgtctt tgactacaga gtattactat ccactacaga
      481 acgcaataat cgagttctac actgaatact ataagactaa ctccatcaat gaaaaaatga
      541 acaaattaga gaataagtat atcgatgcat atcacgttat tttcaaagaa ggtaatctta
      601 acggtgagtg gagtataaat gatgttaatg ccgtttctaa aattgctgct aacgctgtta
      661 acggtattgt tacattcaca c
//
