LOCUS       sequence-132369-        5710 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF 
            attenuator-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 60
FEATURES             Location/Qualifiers
     source          1..5710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     primer_bind     complement(1171..1190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1172..1190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1200..1217)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1200..1216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1209..1231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1358..1813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1445..1464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1655..1676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1839..1943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1944..2012,2013..2804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2162..2181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3827..4777)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4825..5047
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcgagtgaat aaaaggtttt gggcagcgcg ccaatggcgg cgcgtatgtc
     1021 cagggataaa ggcgtccagc ggtgcgtaag caccgccggg cgcttttttt ttgcgcgaat
     1081 tcccaattcg gttcacccgg gcatatggca agaactgcag atatccatgg taccgagctc
     1141 ggatccgtcg acaagcttat cgccaattcg ccctatagtg agtcgtatta cgcgcgctca
     1201 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1261 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1321 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1801 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1861 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1921 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1981 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2041 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2101 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2161 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2221 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2281 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2341 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2401 tgggggatca tgtaactcgc cttgatcgct gggaaccgga gctgaatgaa gccataccaa
     2461 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2521 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2581 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2641 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2701 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2761 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2821 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2881 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2941 cgtcagatct ctgttcgggg gttcactgac gctgcgtgac tacgatggtc aggttgcgga
     3001 ggctatggcc ctggtacgag cgctgaacaa aatgacgaaa gcaggtatgc ctgaaagcgt
     3061 gcgtattgcc tgaaaacaca acccgctacg ggggagactt acccgaaatc tgatttattc
     3121 aacaaagcca ctttcagtat ggatttgggt gttacggggg ggggttggtg cttaaaatcg
     3181 cgctgaggat ttctgatggt gttaagcggg cggttttgag atgtaaactc gcccgtttaa
     3241 cataatggat cttgcgcgca ccgcccgaac accactcgcc acaaaaaacc gccggaacgt
     3301 ccaaaagtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc
     3361 agaatcgcag atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt
     3421 gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt
     3481 cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt
     3541 gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat
     3601 taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga
     3661 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     3721 tgcatatatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
     3781 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
     3841 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
     3901 cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
     3961 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat
     4021 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
     4081 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
     4141 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
     4201 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
     4261 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
     4321 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
     4381 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
     4441 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
     4501 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
     4561 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
     4621 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
     4681 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     4741 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     4801 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     4861 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     4921 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     4981 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     5041 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     5101 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     5161 gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt
     5221 cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac
     5281 aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg
     5341 ggcttctcag ggcgttttat ggcgggtctg cggagtggtg aatccgttag cgaggtgccg
     5401 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     5461 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     5521 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
     5581 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     5641 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     5701 cagcctcgcg
//
