LOCUS       sequence-132377-         810 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  flySTARR-seq screening vector with DSCP core promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pSTARR-seq_fly
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      240..249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          255..321
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(369..397)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc ccgggctcga gatctgcgat
       61 cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc
      121 agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga
      181 ataagccaac tttgaatcac aagacgcata ccaaacggca ttccggtact gttggtaaag
      241 ccaccatgga aaaggtaggt tcaaccactg atgcctaggc acaccgaaac gactaaccct
      301 aattcttatc ctttacttca ggccaaagct taagcatggc tagcaaagga gaagaactct
      361 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaacggc cacaagttct
      421 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctg aagttcatct
      481 gcactactgg caaactgcct gttccctggc caacactagt cactactctg tgctatggtg
      541 ttcaatgctt ttcaagatac ccggatcata tgaaacggca tgactttttc aagagtgcca
      601 tgcccgaagg ttatgtacag gaaaggacca tcttcttcaa agatgacggc aactacaaga
      661 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      721 ttgacttcaa ggaagatggc aacattctgg gacacaaatt ggaatacaac tataactcac
      781 acaatgtata catcatggca gacaaacaaa
//
