LOCUS       sequence-132420-         784 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  flySTARR-seq screening vector with hsp70 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pSTARR-seq_fly-hsp70
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      218..227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          233..299
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(347..375)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc ccgggctcga gatctgagcg
       61 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
      121 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      181 atctgcagaa gcttggcatt ccggtactgt tggtaaagcc accatggaaa aggtaggttc
      241 aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg
      301 ccaaagctta agcatggcta gcaaaggaga agaactcttc actggagttg tcccaattct
      361 tgttgaatta gatggtgatg ttaacggcca caagttctct gtcagtggag agggtgaagg
      421 tgatgcaaca tacggaaaac ttaccctgaa gttcatctgc actactggca aactgcctgt
      481 tccctggcca acactagtca ctactctgtg ctatggtgtt caatgctttt caagataccc
      541 ggatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      601 aaggaccatc ttcttcaaag atgacggcaa ctacaagaca cgtgctgaag tcaagtttga
      661 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gacttcaagg aagatggcaa
      721 cattctggga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      781 caaa
//
