LOCUS       sequence-132575-         950 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1084
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(59..161)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(162..193)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     241..257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     241..257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(400..475)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(496..845)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
ORIGIN
        1 ccatgttgga atttaatcgc ggcttcaagc aagacgtttc ccgttgaata tggctcattt
       61 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
      121 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag cctccggtcg
      181 gaggcttttg actttctgct atggaggtca ggtatgattt aaatggtcag tattgagcct
      241 caggaaacag ctatgacatc aagctgacta gataatctag ctgatcgtgg accgatcata
      301 cgtataatgc cgtaagatca cgggtcgcag cacagctcgc ggtccagtag tgatcgacac
      361 tgctcgatcc gctcgcaccg ctagcaaatt tcacaaaaag caccgactcg gtgccacttt
      421 ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct aaaaccgtgg
      481 tgcctatggt agcgcaagac atctcgcaat aggaggtgtt tatatagggg caacacaaga
      541 ggcggcagac atctcgtgag tttgacagag tagtataaag agtgtgtgtg agagggtgta
      601 ggcatttcat aacatatttt ttgtggaata attttggaaa aaaggtgttt ttttaataat
      661 agcttttata agagtttctc aaaaattgca tgcttttttt gaaaaggttg acaaattata
      721 gtagtactaa agttgctatt ttcaagtctc agcagaaaat tcacaactaa aaataaaatt
      781 tgcaaaacag agtgtgcgcc tttaaggtgt acagtttttt attcctttat tgctagtttt
      841 ttttgaagct tggatcgacg agagcagcgc gactggatct gtcgcccgtc tcaaacgcaa
      901 ccctccggcg gtcgcatatc attcaggacg agcctcagac tccagcgtaa
//
