LOCUS       sequence-132625-        5204 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mEos2 photoconvertible fluorescent protein fused to the N-terminus 
            of centrin-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Sillibourne JE, Specht CG, Izeddin I, Hurbain I, Tran P, Triller A, 
            Darzacq X, Dahan M, Bornens M
  TITLE     Assessing the localization of centrosomal proteins by PALM/STORM 
            nanoscopy.
  JOURNAL   Cytoskeleton (Hoboken). 2011 Nov;68(11):619-27. doi: 
            10.1002/cm.20536. Epub 2011 Oct 18.
  PUBMED    21976302
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.20536"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2011-11"; volume: 
            "68"; issue: "11"; pages: "619-27"
COMMENT     Sequence Label: pmEos2-C1-centrin-1
FEATURES             Location/Qualifiers
     source          1..5204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..678
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    1387..1508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1424..1443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1478..1497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1515..1970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1652..1673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1864..1883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1997..2101
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2103..2460
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2103..2294
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2126..2146)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2311..2446
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2495..3289
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2495..3289
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2549..2568)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3159..3178
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3477..3496)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3521..3568
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3897..4485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4386..4405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4660..4963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4964..5167
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5118..5138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg
       61 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
      121 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
      181 ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag
      241 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
      301 tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
      361 tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
      421 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct
      481 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
      541 gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc
      601 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
      661 cctgacaatg ccagacgact gtacaagtcc ggactcagat ctcgagctca agcttcgaat
      721 tctgcagtcg acggtaccat ggcttccggc ttcaagaagc ccagcgctgc ctccaccggc
      781 caaaagagaa aggtggcacc taagcccgag ctcactgagg atcagaagca agaagttcgg
      841 gaagcatttg acctcttcga cgtggacgga agtgggacca tcgacgcgaa ggagctgaag
      901 gtggccatga gagcgctggg cttcgaaccc aggaaggaag agatgaagaa aatgatctcc
      961 gaggtggaca gggaaggcac ggggaagatc agcttcaatg acttcctggc cgtgatgacg
     1021 cagaagatgt ccgagaagga caccaaagaa gaaatcctga aggccttcag gctctttgat
     1081 gacgatgaga ccgggaagat ctcgttcaaa aacctgaagc gtgtggccaa cgagctgggg
     1141 gagaacctca cggatgagga gctgcaggag atgatcgacg aagctgatcg ggatggggac
     1201 ggcgaagtga acgaggagga gttccttcgg atcatgaaga agaccagcct ttactgagga
     1261 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     1321 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1381 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1441 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1501 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     1561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     1621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     1741 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     1801 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     1861 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     1921 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     1981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2101 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2281 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2341 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2401 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2461 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     2521 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     2581 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     2641 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     2701 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     2761 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     2821 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     2881 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     2941 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     3001 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     3061 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     3121 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     3181 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     3241 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     3301 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     3361 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     3421 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     3481 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     3541 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     3601 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     3661 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     3721 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     3781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4021 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4561 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     4621 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4681 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4741 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4801 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4861 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4921 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4981 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     5041 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     5101 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     5161 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggt
//
