LOCUS       sequence-132689-         938 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway compatible human expression vector that adds a ""flexible"" 
            3XFLAG,V5,YFP N terminus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Mita P, Lhakhang T, Li D, Eichinger DJ, Fenyo D, Boeke JD
  TITLE     Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for 
            High-Throughput IP.
  JOURNAL   Biol Proced Online. 2016 Aug 15;18:16. doi: 
            10.1186/s12575-016-0046-x. eCollection 2016.
  PUBMED    27528826
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Proced
            Online."; date: "2016-08-15"; volume: "18:16. doi"; pages: " 
            10.1186/s12575-016-0046-x. eCollection 2016"
COMMENT     Sequence Label: HuEV-A
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..75
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             82..153
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             154..195
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    196..229
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(232..234,235..237,238..750)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(277..298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(538..557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     885..906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgcttcgct ctctgctcct cctgttcgac agtcagccgc agaagttctt attctctaga
       61 aagtatagga acttcacaat ggactacaag gacgacgacg acaaggatta taaagatgat
      121 gatgataaag actataagga tgacgatgac aaaggcaagc ccatccccaa ccccctgctg
      181 ggcctggaca gcacctaccg ttcgtataat gtatgctata cgaagttatc catggtgagc
      241 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      301 aacggccaca agttcagcgt gtccggtgag ggcgagggcg atgccaccta cggcaagctg
      361 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      421 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      481 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      541 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      601 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      661 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
      721 gccaacttta agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
      781 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      841 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      901 ttcgtgaccg ccgccgggat cactctcggc atggacga
//
