LOCUS       sequence-132696-         873 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression of GEMIN6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     Dreyfuss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1+ GEMIN6 myc HIS
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             638..667
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             683..700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(723..740)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    729..840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gttaagctta tcatgagtga atggatgaag aaaggcccct tagaatggca
      121 agattacatt tacaaagagg tccgagtgac agccagtgag aagaatgagt ataaaggatg
      181 ggttttaact acagacccag tctctgccaa tattgtcctt gtgaacttcc ttgaagatgg
      241 cagcatgtct gtgaccggaa ttatgggaca tgctgtgcag actgttgaaa ctatgaatga
      301 aggggaccat agagtgaggg agaagctgat gcatttgttc acgtctggag actgcaaagc
      361 atacagccca gaggatctgg aagagagaaa gaacagccta aagaaatggc ttgagaagaa
      421 ccacatcccc atcactgaac agggagacgc tccaaggact ctctgtgtgg ctggggtcct
      481 gactatagac ccaccatatg gtccagaaaa ttgcagcagc tctaatgaga ttattctgtc
      541 gcgtgttcag gatcttattg aaggacatct tacagcttcc caagaattct gcagatatcc
      601 agcacagtgg cggccgctcg agtctagagg gcccttcgaa caaaaactca tctcagaaga
      661 ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
      721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      841 gcattgtctg agtaggtgtc attctattct ggg
//
