LOCUS       sequence-132796-       10097 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-VCPIP1
FEATURES             Location/Qualifiers
     source          1..10097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             359..873
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(949..1548)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1032..1052)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1529..1548
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    1560..2112
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1562..2129
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1722..1741)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1951..1968
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    2142..2166
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5836..5860)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5876..5902)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5915..5938)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5938..5947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5960..6376)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5976..5992)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6008..6030)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6443..6784
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             6848..7364
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(7564..7583)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7711..7733)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7927..7946)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8046..8068
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8106..8124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8192..8296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8297..8365,8366..9157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8515..8534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9328..9916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9817..9836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10070..10087
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa
      241 ctatttcagg agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac
      301 ttaatattcg actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat
      361 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      421 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgacg
      481 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      541 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      601 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      661 tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      721 cttaccacag atatcctgtt tggcccatat tctgctgtct ctctgttcct aaccttgatc
      781 tgaacttctc tattctcagt tatgtatttt ccatgccttg caaaatggcg ttacttaagc
      841 tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa
      901 aatcttttat tttatcgata agcttggctg caggtcgaca tcgatgcatc aggcaccggg
      961 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     1021 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     1081 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     1141 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     1201 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     1261 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     1321 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     1381 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     1441 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     1501 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg ttgtggccat
     1561 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
     1621 tttttaacct cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca
     1681 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
     1741 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
     1801 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     1861 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     1921 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     1981 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     2041 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     2101 ggccagtaac gttagggggg gggggcggaa ttcgttatcg aaccactttg tacaagaaag
     2161 ttgggtatca agagtgatcc attggctcag ttgtgttagt catctcagca tcttgactat
     2221 ccatctcttc aagctcctcc acggaatttc ccctttgtgc ttttgacctt gtggcaaagg
     2281 ctgctcccag tgcatctgct acacagccat cagttgtttc tgtattcaaa ttttcagtac
     2341 caccagttaa aggaaaagat tcaggcaaac cagtctgaag actcccagac tttgcagaag
     2401 aactcacaac ttctgttttc ctttgaggaa gatcagatgg tgactgctct gtactttgat
     2461 cccgaaggtg cctgtccatt gaagcctgaa tagaagaaac catttcctgc aatttttttc
     2521 gctgggcctc aaccaaatca ggatcaagct gcctgccgtc tctcattgtt actactccag
     2581 gagctattcg aataagctca ctattactag aagaagctgt gaatacagaa ggctctgttt
     2641 tagtggaact attactaaag tctgtccctg tattatgctt tcttacaact gaagtttccc
     2701 tagctcttgg atcaaggtgt gggttaccag atgcagttcc taaagaatgc ccttttccct
     2761 gaaaggcagt tacgttatga agttgctcag atttcttttt cactactcca ccactaccta
     2821 gttttaatag cccatgtgag ggacttgatt ccctacttct tgtagtagcc tctgctcgaa
     2881 cctgacttac agcctcttta actaaatctt caacatcagg accaatgggg aaatgtcctg
     2941 cagcatccac acacaattcc agtctatctt cagaagcatt atagacaaag gttttgccag
     3001 gcagatgtgg aaaagtacaa tgcttgccat cagccatacc catggttttc ttcataatac
     3061 tgtagaatac accaccctgc tgaaacatgt gaggaagtcc ctttgcataa gaccatacat
     3121 cttctcccat taatgttgct aaaagacaca aggagtacat ttctttttca gcttgctcct
     3181 gaagttcctt agatgacagt ttaccagtaa cagcaatatc ttcttgtttc acagtgtggg
     3241 ctgagtgtgc tgcagcagac tgaccacctt cagctttact ttttagaatt tctattgtaa
     3301 ttctgtcgcc atgctgtaaa ggaactggtt ccttttccat tcctgcctgt ggtggcatta
     3361 actctttagg aggaaacccg tatcgaatac actgtaaata tggaggaatg ttgaattctc
     3421 tggctatact ttcctgaagt tcaaaaaagg ttgttgaaga cttaagggta accatggact
     3481 gtcgtccatc attagttgtg attcggatct tcttctcctt agaagttgtc ggtgaatagg
     3541 gagccttggt aggtgtagca ggtgcagagg atggaccatc acgaatggta ctgggagaaa
     3601 cagtcctggg ttgccctttt tgttcttgtt ttaacttctc tgttttccgt ttctgcatta
     3661 cagaagcctg ttccgtaata ttctgttgaa tagttctttc agttttactc atgtttaact
     3721 cctccttgtg caaagttttt gttttctgtc cagtaagaat aattttagtt gggagctgag
     3781 actctgattc ttgtccttga acatctccaa ctctttgggc gtgagcacca tctatattta
     3841 caggagtata atcatcggga ttctttttgg cagtctgatg caatatagtg tggacaactt
     3901 tctgaactag gatttcactc ccaaattcac ctggaaagtg cttggtaaca agtttcattg
     3961 caacatcgta aacattacta ttcaaagatg aatcactttc atactggaac caatatactg
     4021 tttctctgac cactcttcca ccccattcta aagtaatagg gaaagcttct ggtagattgt
     4081 catactcctt accatcccaa aagtgtttga atccacaacc acatttgcca ccagtggacc
     4141 tagaattagt tctgtctcca tccaaataca caatagaccc atctcctctg acctttcgca
     4201 cagatgtgcc atggcaccaa ttacaagctg ttaggttact cattccatag tctggtacca
     4261 gaacatcttt cactgaatca tatgagcaaa ccaaattgtt caagggaaag ctgtaatttt
     4321 tgtcagtcct cagctgtcca tgagtacttt ttgccaggtt atacaatttc cctccaggag
     4381 ccaaccactc tggaggaaca tgaagttcag aaagggcacc acagagcaaa catttgtgaa
     4441 ggcgattatc cattactgct tttttagcag ctgctgtgac ttcctcaggc tgaactccta
     4501 tcactccagt ccttctgtag aaatactgat ggacatcagc aaccaaacta ggatggatac
     4561 catgtttgtc cataaagact tcttccatag cagcaacaag cctaagtaag tatttatctt
     4621 gcaaacttct gtcacctcca ataacacaac caccatcctc ttcaagtttt atgtactttt
     4681 taataaggtc ctgaggcaca ccccatgctt taggaagcaa attcataggc agtttgggca
     4741 aagcagcccc ttttatgcct accaagggga tataatggtt tctaccggag ctgctccatg
     4801 caatacagat tggtttgttc aaatgaccat ctttcccagt gcacttctct gcagggatga
     4861 gcccaggtag aaaggtggct gaataatcac cagagcttct catgccactg agggaatcta
     4921 acagaataat aggacgatgt agcacattgg caagaccaaa tatgtggata ttcctcaggc
     4981 ccaagggaac accctcaggt ggtacaaaca gagggtcaca ctcattgata atgtcctccc
     5041 actcagcagc atcaatgaag tcatggaaca gagcttgata tcgggccagg tgctgctgaa
     5101 agtgctgttt aagattctct cttaaggcat gccagaagag ctctcggcct actagagccc
     5161 gagacacagc atgcaccaag cagtgtccat ccccgtccac atgcactgga atgagacatt
     5221 cctggctttt attggcccgc ttaatgtcct ccagagtgtc atgcaaatac aggaggcttc
     5281 cggagcggtc cttgccatag cccacagtgt taacatgctc tggttctatg aggaaggcgc
     5341 ggtcacccag taaggcgcaa tcgaacagtt cgccctggtt catgtcccgg agaagcttgg
     5401 cccggcctgt ctgtttgtcc attccatagc gagctaatat gggcgacaac aatttgcagt
     5461 gatagttgga aaggcccatc acctttacca gttccgtgtt cttcttgggt gcccccgtaa
     5521 ccccgagcag cgcgttccgc agcaggttgt gtagcactac gtccgggtcg gtcacctcct
     5581 caacccccag cagctgttgc tgctcgtgcc gctggccgca ctcggtacac tcgatgctga
     5641 cagaaccgga ggccgggaaa aatagacgcg cctgacactt cggatccggg cagctcccgg
     5701 aaaggattct ccggtctctc cgcttcaaaa gcccccccga agcagccgcc gacgccaagg
     5761 acgacggagt ctgtggagcc tcagggggag gaggtggcgg cggcaacgga ggcggcggcg
     5821 gcggcggctg agacatgcca acttttttgt acaaacttgt ttgataacct cgagcagcgt
     5881 aatctggaac atcgtatggg tatcctccat cgagcttgtc atcgtcatcc ttgtagtcca
     5941 tggtggcaga tctaattccg gcgcctagag aaggagtgag ggctggataa agggaggatc
     6001 gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag
     6061 gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt
     6121 cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct
     6181 tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt
     6241 taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg
     6301 actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag
     6361 tggtaacagt ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac
     6421 acagaacgat gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa
     6481 gcaaaaattc agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat
     6541 atcctgacgg ggtcggattc cacatcgact cccttcctca ggtcgggcca caaaaacggc
     6601 ccccaaagtc cctgggacgt ctcccagggt tgcggccggg tgttcagaac tcgtcagttc
     6661 caccacgggt ccgccagata cagagctagt tagctaacta gtacagacgc aggcgcaaac
     6721 attagatgcc ggcacaaaca cgcacaaaga cagagacaga cacgaaacga ccgctggcca
     6781 gcttacctcc cggcgggggg gtcggtggtc cctgggcagg ggtctccaaa tctcggtgga
     6841 acctccaaat gaaagacccc cgaggtgggc agtcaatcaa tctgaggaga ccctcccaag
     6901 gatcagcgag tccacgattc ggatgcaaac agcaagaggc tttattggga atacgggtac
     6961 ccgggcgacg cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta
     7021 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
     7081 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
     7141 tagaaactgc tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg
     7201 gcaggaactg cttaccacag atatcctgtt tggcccatat tctgctgtct ctctgttcct
     7261 aaccttgatc tgaacttctc tattctcagt tatgtatttt ccatgccttg caaaatggcg
     7321 ttacttaagc tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga
     7381 gactaaataa aatcttttat tttatctatg gctcgtactc tataggcttc agctggtgat
     7441 attgttgagt caaaactaga gcctggacca ctgatatcct gtctttaaca aattggacta
     7501 atcgcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     7561 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     7621 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     7681 tctccttgca tgcaccattc cttgcgccgt cgttttacaa cgtcgtgact gggaaaaccc
     7741 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     7861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     7921 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     7981 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8041 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8101 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8161 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8221 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8281 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8341 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8401 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8461 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8521 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8581 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8641 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8701 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8761 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8821 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8881 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8941 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9001 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9061 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9121 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9181 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9241 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9961 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10021 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    10081 gagcgaggaa gcggaag
//
