LOCUS       sequence-132983-         891 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; N-term non-cleavable single tag; contains DHFR 
            gene in BamHI-NgoMIV cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-DHFRHIS
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             536..553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(637..655)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      651..698
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        763..854
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attggaattc aataattttg tttaacttta agaaggagat atacatatgg gatccatcag
       61 tctgattgcg gcgctagcgg tagatcgcgt tatcggcatg gaaaacgcca tgccatggaa
      121 cctgcctgcc gatctcgcct ggtttaaacg caacacctta aataaacccg tgattatggg
      181 gcgccatacc tgggaatcaa tcggtaggcc tttgcccggc cgcaaaaata ttatcctcag
      241 cagtcaaccc gggaccgatg atcgggttac ctgggttaaa tcggtcgacg aagccatcgc
      301 ggcgtgtggt gacgtaccag aaatcatggt gattggcggc ggacgcgttt atgaacagtt
      361 cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgat gcagaagtgg aaggcgacac
      421 ccattttccg gattacgagc cggatgactg ggaatcggta ttcagcgaat tccacgatgc
      481 tgatgcgcag aactcgcata gctattgttt cgaaatcctc gagcgtcgtg ccggccacca
      541 ccaccaccac cactaatgta caaagcttgg taccacgcgt gcgcgctgat ccggctgcta
      601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      661 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatcgg
      721 gattagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
      781 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      841 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc t
//
