LOCUS       sequence-133260-        1060 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: FLAG-Mkk7-JNK2 fusion-pcw107
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(254..265)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ggctcctcgt tgaccgaatc accgacctct ctccccaggg gctagcatcg atggatcaac
       61 aagtttgtac aaaaaagttg gcggcggcgg cggcggcacc atggactaca aagacgatga
      121 tgacaaaatg gcggcgtcct ccctggagca gaagctgtcc cgcctggaag ccaagctgaa
      181 gcaggagaac cgtgaggccc gcaggaggat cgacctcaac ttggatatca gcccacagcg
      241 gcccaggccc accctgcaac tcccactggc caacgatggg ggcagccgct caccatcctc
      301 agagagctcc ccacagcacc ctacaccccc cacccggccc cgccacatgc tggggctccc
      361 atcaaccttg ttcacaccgc gcagtatgga gagcatcgag attgaccaga agctgcagga
      421 gatcatgaag cagacagggt acctgactat cgggggccag cgttatcagg cagaaatcaa
      481 tgacttggag aacttgggtg agatgggcag tggtacctgt ggtcaggtgt ggaagatgcg
      541 gttccggaag acaggccaca tcattgctgt taagcaaatg cggcgctctg ggaacaagga
      601 agagaataag cgcattttga tggacctgga tgtagtactc aagagccatg actgccctta
      661 catcgttcag tgctttggca ccttcatcac caacacagac gtctttattg ccatggagct
      721 catgggcaca tgtgcagaga agctgaagaa acgaatgcag ggccccattc cagagcgaat
      781 cctgggcaag atgactgtgg cgattgtgaa agcactgtac tatctgaagg agaagcatgg
      841 cgtcatccat cgcgatgtca aaccctccaa catcctgcta gatgagcggg gccagatcaa
      901 gctctgtgac tttggcatca gtggccgcct tgttgactcc aaagccaaaa cacggagtgc
      961 tggctgtgct gcctatatgg ctcccgagcg catcgaccct ccagatccca ccaagcctga
     1021 ctatgacatc cgagctgatg tgtggagcct gggcatctca
//
