LOCUS       sequence-133378-         815 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression vector for the analysis of miR-31-19-alpha-193a
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Shen J, Xia W, Khotskaya YB, Huo L, Nakanishi K, Lim SO, Du Y, Wang 
            Y, Chang WC, Chen CH, Hsu JL, Wu Y, Lam YC, James BP, Liu X, Liu CG,
            Patel DJ, Hung MC
  TITLE     EGFR modulates microRNA maturation in response to hypoxia through 
            phosphorylation of AGO2.
  JOURNAL   Nature. 2013 May 16;497(7449):383-7. doi: 10.1038/nature12080. Epub 
            2013 May 1.
  PUBMED    23636329
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12080"; journalName: "Nature"; date: "2013-05-16- 
            16"; volume: "497"; issue: "7449"; pages: "383-7"
COMMENT     Sequence Label: pMIR-31-192-193a-Reporter
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     170..187
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     171..187
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      181 ggccagtgcc aagctagcgg ccgcatacaa aaaaccaaca cacagatcca atgaaaataa
      241 aagatccttt attaagcttt gggtctttgc tgacctatga ggcaagatga ctagtgcgca
      301 gctttcatca ctcgagcaat ttggactttc cgcccttctt ggcctttatg aggatctctc
      361 tgatttttct tgcgtcgagt tttccggtaa gacctttcgg tacttcgtcc acaaacacaa
      421 ctcctccgcg caactttttc gcggttgtta cttgactggc gacgtaatcc acgatctctt
      481 tttccgtcat cgtctttccg tgctccaaaa caacaacggc ggcgggaagt tcaccggcgt
      541 catcgtcggg aagacctgcc acgcccgcgt cgaagatgtt ggggtgttgt aacaatatcg
      601 attccaattc agcgggggcc acctgatatc ctttgtattt aattaaagac ttcaagcggt
      661 caactatgaa gaagtgttcg tcttcgtccc agtaagctat gtctccagaa tgtagccatc
      721 catccttgtc aatcaaggcg ttggtcgctt ccggattgtt tacataaccg gacataatca
      781 taggtcctct gacacataat tcgcctctct gatta
//
