LOCUS       sequence-133505-         836 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector 
            with DS2 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Rugbjerg P, Myling-Petersen N, Sommer MO
  TITLE     Flexible metabolic pathway construction using modular and divisible 
            selection gene regulators.
  JOURNAL   Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. 
            Epub 2015 Aug 21.
  PUBMED    26303342
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-09"; volume: "31:189-97. doi"; pages: " 
            10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21"
COMMENT     Sequence Label: pDS2-aurZ
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    41..544
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     586..604
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(642..664)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    785..818
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc atcacgtgct ataaaaataa
       61 ttataattta aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa
      121 ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac
      181 aaatactacc ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc
      241 ttgtctgtgt agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa
      301 tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc
      361 tttttgttga aattttttaa acctttgttt attttttttt cttcattccg taactcttct
      421 accttcttta tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag
      481 taaattccca aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc
      541 gatccgtcct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      601 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
      661 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
      721 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atacagcttt cgacaaccct
      781 taatataact tcgtataatg tatgctatac gaagttatta ggtctagaga tctgtt
//
