LOCUS       sequence-133651-         707 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  For mammalian expression of an N-terminally triple FLAG-tagged and 
            C-terminally triple AU1-tagged full length human GATA6 with triple 
            alanine mutations on aminoacids#33,#34 and #37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger 
            DA, Janes KA
  TITLE     TNF-insulin crosstalk at the transcription factor GATA6 is revealed 
            by a model that links signaling and transcriptomic data tensors.
  JOURNAL   Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
  PUBMED    27273097
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-06-7"; volume: "9(431):ra59. doi"; pages: " 
            10.1126/scisignal.aad3373"
COMMENT     Sequence Label: pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..64
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gccggtccgg atcatggact acaaggatga cgatgacaag gactacaagg atgacgatga
       61 caaggactac aaggatgaca atgacaagat ggccttgact gacggcggct ggtgcttgcc
      121 gaagcgcttc ggggccgcgg gtgcggacgc cagcgactcc agagcctttc cagcgcggga
      181 gcccgccgcg ccgcctgccc ccatctcttc ctcgtcctcc tcctgctccc ggggcggaga
      241 gcggggcccc ggcggcgcca gcaactgcgg gacgcctcag ctcgacacgg aggcggcggc
      301 cggacccccg gcccgctcgc tgctgctcag ttcctacgct tcgcatccct tcggggctcc
      361 ccacggacct tcggcgcctg gggtcgcggg ccccggggca acctgtcgag ctgggaggac
      421 ttgctgctgt tcactgacct cgaccaagcc gcgaccgcca gcaagctgct gtggtccagc
      481 ctcggcgcca agctgagccc cttctcaccc gagcagctgg aggagatgta ccataccctc
      541 gccgctctct ccatcttggt ccggccgcct actactgcgc gcctgtcggc ttcttgctct
      601 ctgcgtttgc ggcgttttct cccgcggcgg cggacttctt ctaggttttg tgcattattt
      661 aacacatcgt ttcttggatc agccattctt gaaccaattg tggagtg
//
