LOCUS       sequence-134035-         965 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of Acetobacter aceti 1023 PurE and PurK in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK174
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             570..578
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 taagaaggag atatacatat gatgagcgaa accgcccccc tgccctctgc ttccagtgct
       61 ttagaagata aagcagcctc tgcccccgtt gtgggcatta tcatgggcag ccagtcagac
      121 tgggaaacca tgcgccatgc agacgcattg ctgacagagc tggaaattcc gcatgaaacc
      181 ctcattgttt cagcacatcg tacgccagac cggttggcgg attatgcgcg tacggcagcc
      241 gaacgcggcc tgaatgtgat tattgctggg gccggtggag ctgcacatct gccgggtatg
      301 tgtgcggcat ggacacgcct gcccgtgctg ggtgttccag tagaatctcg cgctcttaaa
      361 ggcatggaca gcctgctttc cattgtgcag atgcccggcg gcgtgcctgt gggcactttg
      421 gctattgggg catccggcgc caaaaatgcc gccttgctgg ccgcctccat tcttgcactg
      481 tataacccgg cccttgccgc acgccttgaa acatggcgcg cattgcagac ggcctctgtc
      541 cccaactctc ccattaccga agataaatga cctccttaca ttctccgctt aagcccaatg
      601 ccgttattgg cattgtgggg ggtggccagc ttggccgcat gtctgctgtt gctgccgcgc
      661 gccttggttt tcgcacgcat attcttacgc cagaggccaa cggcccggcg gctcaggtct
      721 ctcacgccgt aacatgcggc aagtatgatg atccgacagc actggatgcc tttgcccgcg
      781 ctgttgatgt tgttaccttt gaatttgaaa acatcagcgc agatgcactg gaccgcctag
      841 ccaaacactg ccctgttcat ccttcggggc atattctgcg catcagtcag gaccgattgg
      901 cagaaaaaac tttttttgct tctgcgggta ttccactcgc gccatggcat cctgttaccg
      961 atgtc
//
