LOCUS       sequence-134069-         680 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express Streptococcus pyogenes dCas9 (D10A, H840A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1003
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..167)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(225..248)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 agattttttt tgatactgtg gcggtctgta tttcccagaa ccttgaactt tttagacgga
       61 accttatatt catcagtgat caccgcccat ccgacgctat ttgtgccgat agctaagcct
      121 attgagtatt tcttatccat tgcattggat tggaagtaca ggttttcctc gatcccgagg
      181 ttgttgttat tgttattgtt gttgttgttc gagctcgaat tagtctgcgc gtctttcagg
      241 gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac
      301 cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccata
      361 gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca
      421 cccagcggtt tgtctttatt aaccgcttcc agaccttcat cagtcagcag atagttttcg
      481 aggaactctt ttgccagctc tttgttcgga ctggcggcgt taatacctgc gctcagcacg
      541 ccaacgaacg gtttggatgg ttgacccttg aaggtcggca gtaccgttac accataattc
      601 actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg
      661 cctttattaa aggcagcttc
//
