LOCUS       sequence-134139-        3718 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE(H59D) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK346
FEATURES             Location/Qualifiers
     source          1..3718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             823..831
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(847..864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(847..863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(856..878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1076..1531
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1163..1182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1373..1394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1548..1567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1667..1689
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1727..1745)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1813..1917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1918..1986,1987..2778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2136..2155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2949..3537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3438..3457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3691..3708
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcctccg
      241 ttacagactt taaaaaaccg gatagatacc caccgtgagc gaaaccgccc ccctgccctc
      301 tgcttccagt gctttagaag ataaagcagc ctctgccccc gttgtgggca ttatcatggg
      361 cagccagtca gactgggaaa ccatgcgcca tgcagacgca ttgctgacag agctggaaat
      421 tccgcatgaa accctcattg tttctgcaga tcgtacgcca gaccggttgg cggattatgc
      481 gcgtacggca gccgaacgcg gcctgaatgt gattattgct ggggccggtg gagctgcaca
      541 tctgccgggt atgtgtgcgg catggacacg cctgcccgtg ctgggtgttc cagtagaatc
      601 tcgcgctctt aaaggcatgg acagcctgct ttccattgtg cagatgcccg gcggcgtgcc
      661 tgtgggcact ttggctattg gggcatccgg cgccaaaaat gccgccttgc tggccgcctc
      721 cattcttgca ctgtataacc cggcccttgc cgcacgcctt gaaacatggc gcgcattgca
      781 gacggcctct gtccccaact ctcccattac cgaagataaa tgacctcctt acattctaag
      841 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      901 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      961 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     1021 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     1081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1261 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     1321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1381 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     1441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1501 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     1561 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1621 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     1681 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1741 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1801 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1861 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1921 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1981 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2041 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2101 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2161 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2221 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2281 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2341 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2401 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2461 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2521 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2581 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2641 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2701 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2761 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2821 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2881 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3061 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3601 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3661 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
