LOCUS       sequence-134162-         930 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-DHFRFLAG
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..55)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             552..574
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(617..636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(618..636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atcagcgcgc acgcgtggta cctgtacatt acttgtcatc atcgtccttg tagtcgccgg
       61 cacgacgctc gaggatttcg aaacaatagc tatgcgagtt ctgcgcatca gcatcgtgga
      121 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cggaaaatgg gtgtcgcctt
      181 ccacttctgc atcgatatgc gtcagataca gtttttgcgc ttttggcaag aactgttcat
      241 aaacgcgtcc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
      301 cgtcgaccga tttaacccag gtaacccgat catcggtccc gggttgactg ctgaggataa
      361 tatttttgcg gccgggcaaa ggcctaccga ttgattccca ggtatggcgc cccataatca
      421 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccatggca
      481 tggcgttttc catgccgata acgcgatcta ccgctagcgc cgcaatcaga ctgatggatc
      541 ccatatgtat atctccttct taaagttaaa caaaattatt gagctctccg gagccggagg
      601 gaaaccgttg tggtctccct atagtgagtc gtattaattt cgcgggatcg atgcatggtg
      661 ccaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
      721 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
      781 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattacccca
      841 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
      901 acatggcccg ctttatcaga agccagacat
//
